Early intervention of N-acetylcysteine better improves insulin resistance in diet-induced obesity mice

Autor:	Tsu-Kung Lin, Jiin-Haur Chuang, Chia-Wei Liou, Chia-Shiang Chang, Ching-Yi Lin, Feng-Chih Shen, Shao-Wen Weng, Wei-Shiung Lian, Pei-Wen Wang, Hung-Yu Lin, Cheng-Feng Tsao
Rok vydání:	2018
Předmět:	0301 basic medicine Male medicine.medical_specialty Adipose tissue Type 2 diabetes medicine.disease_cause Biochemistry Acetylcysteine 03 medical and health sciences Mice Insulin resistance Internal medicine Diabetes mellitus medicine Secondary Prevention Animals Obesity Glucose tolerance test medicine.diagnostic_test business.industry Insulin tolerance test food and beverages General Medicine medicine.disease Diet Mitochondria Mice Inbred C57BL 030104 developmental biology Endocrinology Insulin Resistance business Reactive Oxygen Species Oxidative stress medicine.drug
Zdroj:	Free radical research. 52(11-12)
ISSN:	1029-2470
Popis:	Reactive oxygen species (ROS) plays a crucial role in pathogenesis of insulin resistance (IR) and type 2 diabetes. In the United Kingdom, Prospective Diabetes Study and its 10-year post-trial monitoring, a beneficial effect of early optimisation of blood glucose control is clearly demonstrated. In this study, we investigated whether ROS scavenger N-acetylcysteine (NAC) and the time point of intervention can affect IR in a diet-induced obesity mouse model. Male C57B/L6 mice were fed chow diet (CD), high-fat high-sucrose diet (HFD), CD + NAC1-6 (NAC intervention 1st to 6th month), HFD + NAC1-6, and HFD + NAC3-6 (NAC intervention 3rd to 6th month) for a 6-month treatment course. HFD group showed significantly increased body weight (BW) and body fat, decreased motor activity (MA), impaired intraperitoneal glucose tolerance test (IPGTT), and insulin tolerance test (IPITT) throughout the study. HFD + NAC1-6, as compared with HFD group, had increased MA, improved IPGTT and IPITT since first month, followed by decreased BW and body fat. HFD + NAC3-6 group, although showed improved IPGTT and IPITT than HFD group, still had higher BW, decreased MA, and impaired IPGTT and IPITT as compared with HFD + NAC1-6 at the end of the study. NAC significantly increased MA, and ameliorated the HFD-induced mitochondrial and intracellular ROS expression, DNA and protein oxidative damage, and adipose tissue inflammation. We concluded that ROS scavenger can improve IR and chronic inflammation in diet-induced obesity mice. This action is likely better expressed through early intervention. The mechanism is probably through a virtuous circle of suppressed oxidative stress, and increased motor activity, which helps to reduce body fat.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f4a708bf797a8ec10b103868a6dc2d8 https://pubmed.ncbi.nlm.nih.gov/29502477 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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