Circulating metabolites of strawberry mediate reductions in vascular inflammation and endothelial dysfunction in db/db mice(–)
| Autor: | J. David Symons, Chrissa Petersen, Divya Bharat, Brett Ronald Cutler, Jennifer Ellen Mueller, Ji Seok Kim, Pon Velayutham Anandh Babu, Christopher Denetso, Jae Min Cho, Samira Gholami |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty 030209 endocrinology & metabolism Mice Transgenic 030204 cardiovascular system & hematology Fragaria Article Nitric oxide 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Enos Diabetes mellitus Internal medicine medicine Animals Interleukin 8 Vascular Diseases Endothelial dysfunction VCAM-1 Inflammation ICAM-1 biology business.industry Monocyte medicine.disease biology.organism_classification Mice Inbred C57BL Vasodilation Endocrinology medicine.anatomical_structure chemistry Diabetes Mellitus Type 2 Dietary Supplements Endothelium Vascular Cardiology and Cardiovascular Medicine business |
| Popis: | Background Cardiovascular disease is 2-4-fold more prevalent in patients with diabetes. Human studies support the cardiovascular benefits of strawberry consumption but the effects of strawberry on diabetic vasculature are unknown. We tested the hypothesis that dietary strawberry supplementation attenuates vascular inflammation and dysfunction in diabetic mice. Methods Seven-week-old diabetic db / db mice that consumed standard diet ( db / db ) or diet supplemented with 2.35% freeze-dried strawberry ( db / db + SB) for ten weeks were compared to non-diabetic control mice ( db / + ). Indices of vascular inflammation and dysfunction were measured. Endothelial cells (ECs) were isolated from the vasculature to determine the influence of strawberry on them. The effect of metabolites of strawberry on endothelial inflammation was determined by incubating mouse aortic ECs (MAECs) with ±5% serum, obtained from strawberry fed mice (metabolites serum) or standard diet fed mice (control serum) ± 25 mM glucose and 100 μM palmitate. Results db / db mice exhibited an increased monocyte binding to vessel, elevated blood pressure, and reduced endothelial-dependent vasorelaxation compared with db / + mice but each defect was attenuated in db / db + SB mice. The elevation of inflammatory molecules, NOX2 and inhibitor-κB kinase observed in ECs from db / db vs. db / + mice was suppressed in db / db + SB mice. Glucose and palmitate increased endothelial inflammation in MAECs but were normalized by co-incubation with metabolites serum. Conclusions Dietary supplementation of strawberry attenuates indices of vascular inflammation and dysfunction in diabetic db / db mice. The effect of strawberry on vasculature is endothelial-dependent and possibly mediated through their circulating metabolites. Strawberry might complement conventional therapies to improve vascular complications in diabetics. |
| Databáze: | OpenAIRE |
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