Biofilm Production by Enterotoxigenic Strains of Bacillus cereus in Different Materials and Under Different Environmental Conditions
| Autor: | Lilia-Lizette García-Díaz, Yesenia Ramírez-Sandoval, Abigail Pérez-Valdespino, Arturo Ramírez-Peralta, Carlos Ortuño-Pineda, Itzel-Maralhi Cruz-Facundo, Roberto Adame-Gómez, Maria-Cristina Santiago-Dionisio |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
food.ingredient Food industry Bacillus cereus Enterotoxin Microbiology Tryptic soy broth Article biofilm 03 medical and health sciences chemistry.chemical_compound food Virology medicine Agar Food science lcsh:QH301-705.5 030304 developmental biology 0303 health sciences Food poisoning biology 030306 microbiology business.industry fungi Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease chemistry Cereus lcsh:Biology (General) business enterotoxin |
| Zdroj: | Microorganisms Volume 8 Issue 7 Microorganisms, Vol 8, Iss 1071, p 1071 (2020) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8071071 |
| Popis: | Foodborne illnesses, such as infections or food poisoning, can be caused by bacterial biofilms present in food matrices or machinery. The production of biofilms by several strains of Bacillus cereus on different materials under different culture conditions was determined, as well as the relationship of biofilms with motility, in addition to the enterotoxigenic profile and candidate genes that participate in the production of biofilms. Biofilm production of B. cereus strains was determined on five materials: glass, polystyrene, polyethylene, polyvinylchloride (PVC), PVC/glass in three culture media: Phenol red broth, tryptic soy broth, and brain heart infusion broth in two different temperatures (37 ° C and 25 ° C), and in two different oxygen conditions (oxygen and CO2 tension). Furthermore, the strains were molecularly characterized by end-point polymerase chain reaction. Motility was determined on semi-solid agar. The B. cereus strains in this study were mainly characterized as enterotoxigenic strains statistically significant differences were found in the PVC material and biofilm production. Motility was positively associated with the production of biofilm in glass/PVC. The sipW and tasA genes were found in two strains. The results of this study are important in the food industry because the strains carry at least one enterotoxin gene and produce biofilms on different materials |
| Databáze: | OpenAIRE |
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