Next generation sequencing yields complete mitogenomes of Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) (Chondrichthyes: Dasyatidae)
| Autor: | Ching-Hung Chen, Chih-Wei Chang, Philippe Borsa, Shan-Chun Wu, Zi-Han Lin, Kang-Ning Shen, Shiou-Yi Tsai, Yen-Fan Chan, Chung-Der Hsiao |
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| Přispěvatelé: | Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, National Taiwan Ocean University (NTOU), Department of Biology, National Museum of Marine Biology and Aquarium, Checheng, National Museum of Marine Biology and Aquarium, Chung Yuan Christian University, WeThink Biotech Inc., Taoyuan, Taiwan, Ecologie marine tropicale des océans Pacifique et Indien (ENTROPIE [Nouvelle-Calédonie]), Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Ifremer - Nouvelle-Calédonie, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de la Nouvelle-Calédonie (UNC), NTOU |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA DNA sequencing 03 medical and health sciences Paleontology Leopard whipray RNA Transfer biology.animal Stingray Genetics Animals Skates Fish 14. Life underwater Molecular Biology Phylogeny next generation sequencing Base Composition [SDV.GEN]Life Sciences [q-bio]/Genetics biology Phylogenetic tree mitogenome High-Throughput Nucleotide Sequencing Vertebrate Ribosomal RNA biology.organism_classification Chondrichthyes 030104 developmental biology Blue-spotted stingray RNA Ribosomal Evolutionary biology Genome Mitochondrial GC-content |
| Zdroj: | Mitochondrial DNA Part A Mitochondrial DNA Part A, Taylor and Francis, 2016, 27, pp.2613-2614. ⟨10.3109/19401736.2015.1041119⟩ |
| ISSN: | 2470-1394 2470-1408 |
| Popis: | International audience; The Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) are distributed in the Indian and West Pacific Ocean and considered as species complexes based on morphological and molecular evidence. In this study, we used the next-generation sequencing method to decipher the two complete mitogenomes of H. leoparda and N. kuhlii. The assembled mitogenomes had a length of 17,690 bp in H. leoparda and 17,974 bp in N. kuhlii, and showed 78% identity to each other. Both mitogenomes had the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNAs genes and the non-coding control region. The control region, which was 1931-bp long in H. leoparda and 2243-bp long in N. kuhlii was located between the tRNA-Pro and tRNA-Phe loci. The overall GC content was 40.3% in H. leoparda and 39.8% in N. kuhlii. The complete mitogenome sequences of H. leoparda and N. kuhlii provide important molecular data for further phylogenetic and evolutionary analyses of these two stingray species complexes. |
| Databáze: | OpenAIRE |
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