Increase of cortisol levels after temperature stress activates dmrt1a causing female-to-male sex reversal and reduced germ cell number in medaka

Autor: Amaury Herpin, Minoru Tanaka, Martina Regensburger, Mateus Contar Adolfi, Mariko Kikuchi, Manfred Schartl, Peter Fischer
Přispěvatelé: Physiological Chemistry, Biocenter, Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Division of Biological Science, Graduate School of Science, Nagoya University, University of Wuerzburg, Germany and Hagler Institute for Advanced Study and Department of Biology, Comprehensive Cancer Center Mainfranken, University Hospital Würzburg, Texas A&M University System, Deutsche Forschungsgemeinschaft to MS (Scha408/12‐1, 10‐1) and to AH (HE 7135/2‐1). AH was also supported by Crédits Incitarifs Phase 2015/Emergence and by the project AquaCRISPR (ANR‐16‐COFA‐0004‐01), University of Würzburg = Universität Würzburg, This work was supported by grants from the Deutsche Forschungsgemeinschaft to MS (Scha408/12‐1, 10‐1) and to AH (HE 7135/2‐1). AH was also supported by Crédits Incitarifs Phase 2015/Emergence and by the project AquaCRISPR (ANR‐16‐COFA‐0004‐01)., ANR-16-COFA-0004,AquaCrispr,Optimization of the CRISPR/Cas9 knock-in technology and application in salmon and trout(2016)
Rok vydání: 2018
Předmět:
Zdroj: Molecular Reproduction and Development
Molecular Reproduction and Development, Wiley, 2019, Epub ahead of print (10), pp.1-13. ⟨10.1002/mrd.23177⟩
Molecular Reproduction and Development, Wiley, 2019, 86 (10), pp.1405-1417. ⟨10.1002/mrd.23177⟩
ISSN: 1098-2795
1040-452X
DOI: 10.1002/mrd.23177⟩
Popis: In vertebrates, there is accumulating evidence that environmental factors as triggers for sex determination and genetic sex determination are not two opposing alternatives but that a continuum of mechanisms bridge those extremes. One prominent example is the model fish species Oryzias latipes which has a stable XX/XY genetic sex determination system, but still responds to environmental cues, where high temperatures lead to female-to-male sex reversal. However, the mechanisms behind are still unknown. We show that high temperatures increase primordial germ cells (PGC) numbers before they reach the genital ridge, which, in turn, regulates the germ cell proliferation. Complete ablation of PGCs led to XX males with germ cell less testis, whereas experimentally increased PGC numbers did not reverse XY genotypes to female. For the underlying molecular mechanism, we provide support for the explanation that activation of the dmrt1a gene by cortisol during early development of XX embryos enables this autosomal gene to take over the role of the male determining Y-chromosomal dmrt1bY.
Databáze: OpenAIRE
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