Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit
| Autor: | María Nieves Padilla, M. Dolores Sicardo, M. Luisa Hernández, José Manuel Martínez-Rivas, Manuel Mancha |
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| Rok vydání: | 2011 |
| Předmět: |
Fatty Acid Desaturases
FAD2 Linoleic acid Time Factors FAD6 Palmitic Acid Plant Science Horticulture Biology Biochemistry chemistry.chemical_compound Stress Physiological Olea Gene expression Escherichia coli Phosphatidylcholine desaturase Molecular Biology chemistry.chemical_classification Wound Healing Temperature Olive Fatty acid Palmotolinoleic acid General Medicine biology.organism_classification Lipids Oleic acid Olea europaes Enzyme chemistry Fruit Olea desaturase Oleaceae Microsome Oleae |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/j.phytochem.2010.11.026 |
| Popis: | The regulation of microsomal and plastidial oleate desaturases by low and high temperature, darkness, and wounding was investigated. To this end, their gene expression levels and the fatty acid composition was determined in the mesocarp tissue of olive fruit from the Picual and Arbequina varieties subjected to the corresponding stress treatments. Firstly, a plastidial oleate desaturase from olive was cloned and its functional identity was confirmed by overexpression in Escherichia coli. The results showed that temperature and light regulate olive oleate desaturase genes at transcriptional level. However, no correlation between their expression levels and the linoleic acid content in microsomal and plastidial lipids was found. In addition, the involvement of microsomal but not plastidial oleate desaturases in the wounding response of olive fruit mesocarp is demonstrated. The fatty acid analysis revealed the appearance of palmitolinoleic acid only in microsomal lipids, reaching a maximum 3 h after wounding. © 2010 Elsevier Ltd. All rights reserved. This work was supported by Research Projects AGL2004-02060 from MEC (Spain) and P06-AGR-02151 from Junta de Andalucía (Spain). |
| Databáze: | OpenAIRE |
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