Prospective evaluation of rapid point-of-care tests for the diagnosis of acute leptospirosis in dogs
| Autor: | Luca Magna, Francesco Dondi, Mara Battilani, Roberta Troia, S. Frigo, Andrea Balboni, L. Perissinotto, S Zamagni |
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| Přispěvatelé: | Troìa R, Balboni A, Zamagni S, Frigo S, Magna L, Perissinotto L, Battilani M, Dondi F. |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty 040301 veterinary sciences Point-of-care testing Point-of-Care Systems 030106 microbiology Canine leptospirosis Sensitivity and Specificity Prospective evaluation 0403 veterinary science 03 medical and health sciences Dogs Internal medicine Direct agglutination test Agglutination Tests Medicine Animals Leptospirosis Dog Diseases Prospective Studies Prospective cohort study Leptospira General Veterinary business.industry Canine In-clinic diagnostic tests Leptospirosis Microscopic agglutination test Real-time PCR Acute kidney injury 04 agricultural and veterinary sciences medicine.disease Antibodies Bacterial Systemic inflammatory response syndrome Animal Science and Zoology business |
| Zdroj: | Veterinary journal (London, England : 1997). 237 |
| ISSN: | 1532-2971 |
| Popis: | The early diagnosis of acute leptospirosis is still a major challenge in dogs. The aim of this prospective study was to evaluate the suitability of two in-clinic tests detecting anti-leptospiral IgM and IgG antibodies in diagnosing canine leptospirosis. The performances of the two rapid tests were compared to the microscopic agglutination test (MAT) carried out on acute sera and to diagnostic criteria adopted in this study to confirm leptospirosis infection (MAT upon admission, convalescent MAT and quantitative real-time PCR on blood and/or urine). The dogs were enrolled on the basis of reported exposure to known risk factors and clinical presentation (acute kidney injury and/or systemic inflammatory response syndrome with multi-organ damage). Eighty-nine dogs included in the study were sub-grouped on the basis of the results of the diagnostic criteria adopted: (1) confirmed leptospirosis cases (42/89 dogs); (2) negative leptospirosis cases (36/89 dogs); and (3) unconfirmed leptospirosis cases (11/89 dogs). The results supported the usefulness of the two rapid diagnostic tests as a first in-clinic screening tool for suspected leptospirosis; positive results in the in-clinic tests in dogs with suggestive clinical and laboratory signs strongly indicated acute leptospirosis, while negative results required additional diagnostic investigation to exclude the infection. Confirmatory tests recommended for canine leptospirosis are still necessary in addition to the use of rapid in-clinic tests. |
| Databáze: | OpenAIRE |
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