Involvement of Calcium, Reactive Oxygen Species, and ATP in Hexavalent Chromium-Induced Damage in Red Blood Cells
| Autor: | Yang Xiang, Yanni Xiao, Rong Zhang, Zhongjun Li, Lixin Xiang, Xiaojun Deng, Qian Ran |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Chromium
Erythrocytes Physiology Eryptosis chemistry.chemical_element Calcium Cell morphology lcsh:Physiology lcsh:Biochemistry chemistry.chemical_compound Adenosine Triphosphate Humans lcsh:QD415-436 Hexavalent chromium Incubation chemistry.chemical_classification Reactive oxygen species lcsh:QP1-981 Phosphatidylserine RBCs chemistry Biochemistry Toxicity Biophysics Hexavalent chromium (Cr6+) Reactive Oxygen Species Intracellular Signal Transduction |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 34, Iss 5, Pp 1780-1791 (2014) |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | The aim of this study was to investigate the mechanisms of Cr(6+)-induced red blood cells (RBCs) damage.The effect of Cr(6+) exposure on RBCs was evaluated by hemolytic rate and blood gas assays. After exposure to 20 μM Cr(6+), the percentage of phosphatidylserine (PS)-exposing cells, intracellular Ca(2+), reactive oxygen species (ROS), and ATP levels were evaluated, and cell morphology was observed. RBCs were exposed to Cr(6+) in different Ringer solutions to investigate the role of changes of Ca(2+), ROS, and ATP levels in eryptosis and morphology.The Cr(6+)-induced damage of RBCs was dose-dependent. After a 6 h incubation with Cr(6+), RBCs exhibited significant Ca(2+) influx, ROS increase, ATP depletion, and PS exposure, but displayed no obvious change in morphology at this time point. After 24 h Cr(6+) exposure, the RBCs decreased in size, appeared to be spike-like, and had decreased forward scatter. Inhibition of Ca(2+) influx attenuated PS-exposure caused by 6 h Cr(6+) exposure, but did not prevent 24 h Cr(6+) exposure-induced morphological change in RBCs. Inhibition of rapid ATP consumption using adenine significantly ameliorated Cr(6+)-caused PS-exposure at 12 h, 24 h and 48 h, and prevented 24 h Cr(6+) incubation-induced morphological change in RBCs.Cr(6+) can lead to eryptosis. Ca(2+) influx, increased ROS levels, and rapid ATP consumption are closely related to Cr(6+)-induced RBCs damage. Ca(2+) influx plays an extremely important role in Cr(6+)-mediated toxicity. |
| Databáze: | OpenAIRE |
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