Genetically encoded fluorescent indicators for live cell pH imaging
| Autor: | Vladimir I. Martynov, Alexey A. Pakhomov, I. E. Deyev, Alexander G. Petrenko |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology Computer science Intracellular pH Green Fluorescent Proteins Cell Biophysics Biosensing Techniques Computational biology Hydrogen-Ion Concentration Cellular level Diagnostic tools Biochemistry 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Fluorescence Resonance Energy Transfer medicine Humans Fluorescent protein WHOLE ANIMAL Molecular Biology Fluorescent Dyes Signal Transduction |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1862:2924-2939 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2018.09.013 |
| Popis: | Background Intracellular pH underlies most cellular processes. There is emerging evidence of a pH-signaling role in plant cells and microorganisms. Dysregulation of pH is associated with human diseases, such as cancer and Alzheimer's disease. Scope of review In this review, we attempt to provide a summary of the progress that has been made in the field during the past two decades. First, we present an overview of the current state of the design and applications of fluorescent protein (FP)-based pH indicators. Then, we turn our attention to the development and applications of hybrid pH sensors that combine the capabilities of non-GFP fluorophores with the advantages of genetically encoded tags. Finally, we discuss recent advances in multicolor pH imaging and the applications of genetically encoded pH sensors in multiparameter imaging. Major conclusions Genetically encoded pH sensors have proven to be indispensable noninvasive tools for selective targeting to different cellular locations. Although a variety of genetically encoded pH sensors have been designed and applied at the single cell level, there is still much room for improvements and future developments of novel powerful tools for pH imaging. Among the most pressing challenges in this area is the design of brighter redshifted sensors for tissue research and whole animal experiments. General significance The design of precise pH measuring instruments is one of the important goals in cell biochemistry and may give rise to the development of new powerful diagnostic tools for various diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect