[Change of transcription level of photoreceptor-specific CRX gene in the peripheral blood of the participants of an arctic world oceanic international flight]
| Autor: | M. V. Polyakova, A.Yu. Gorbachev, Irina Alchinova, Eduard V. Generozov, M. Yu. Karganov, O. Yu. At’kov, S. G. Gorokhova |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Messenger RNA Retina Retinal Biology Housekeeping gene Cell biology 03 medical and health sciences Ophthalmology chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure chemistry Transcription (biology) medicine Homeobox Humans Gene Transcription factor 030217 neurology & neurosurgery Photoreceptor Cells Vertebrate |
| Zdroj: | Vestnik oftalmologii. 137(2) |
| ISSN: | 0042-465X |
| Popis: | The CRX gene encoding the cone-rod homeobox protein is a specific photoreceptor transcription factor crucial for retinal function. Persons temporarily residing in the Arctic zone during the polar summer may suffer from disturbances associated with extremely high ambient illumination. These environmental changes are mediated by retinal photoreceptors; therefore, it is important to study the expression of retinal genes in order to assess individual capacities of sensory adaptation to polar day conditions. PURPOSE To reveal the dynamics of CRX expression level in humans after a prolonged temporary exposure to polar day conditions. MATERIAL AND METHODS The study included 6 pilots (males from 39 to 69 y.o.) who participated in the Arctic World Oceanic International Flight Sever Vash (West to East, from 62°N 74°E to 72°N 114°E). Samples of peripheral blood for RNA isolation were collected at the start and at the end of the flight. The level of mRNA in the samples was evaluated based on the data of quantitative real-time PCR of the CRX gene, as well as the b2M and TBP housekeeping genes (reference). RESULTS Expression of the CRX gene in the studied group (p 0 after the flight). In one pilot, the level of CRX expression was higher at the start, but it reduced by the end of the circumnavigation flight. CONCLUSIONS The results confirm the hypothesis that the CRX gene is expressed after a prolonged temporary exposure to polar day conditions. It was also revealed that during rapid adaptation, equal changes in the illumination of retinal photoreceptors lead to different individual dynamics of CRX expression. |
| Databáze: | OpenAIRE |
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