Endogenous Retroviruses Transcriptional Modulation After Severe Infection, Trauma and Burn

Autor: Olivier Tabone, Marine Mommert, Camille Jourdan, Elisabeth Cerrato, Matthieu Legrand, Alain Lepape, Bernard Allaouchiche, Thomas Rimmelé, Alexandre Pachot, Guillaume Monneret, Fabienne Venet, François Mallet, Julien Textoris
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Microarray
Neutrophils
viruses
Endogenous retrovirus
Monocytes
Cohort Studies
Transcriptome
0302 clinical medicine
burn
host response
2.1 Biological and endogenous factors
Immunology and Allergy
Aetiology
Original Research
Statistics
Shock
Middle Aged
Shock
Septic
Healthy Volunteers
trauma
Medical Microbiology
embryonic structures
Cohort
Female
Burns
lcsh:Immunologic diseases. Allergy
Adult
Physical Injury - Accidents and Adverse Effects
Immunology
Statistics
Nonparametric
endogenous retroviruses
Sepsis
03 medical and health sciences
Clinical Research
Immunity
Genetics
medicine
Humans
Nonparametric
Gene
Aged
Inflammation
Septic
Septic shock
business.industry
Microarray analysis techniques
medicine.disease
030104 developmental biology
Genetic Loci
Wounds and Injuries
septic shock
lcsh:RC581-607
business
transcriptome
severe inflammatory injuries
030215 immunology
Zdroj: Frontiers in Immunology, Vol 9 (2019)
Frontiers in Immunology
ISSN: 1664-3224
Popis: Although human endogenous retroviruses (HERVs) expression is a growing subject of interest, no study focused before on specific endogenous retroviruses loci activation in severely injured patients. Yet, HERV reactivation is observed in immunity compromised settings like some cancers and auto-immune diseases. Our objective was to assess the transcriptional modulation of HERVs in burn, trauma and septic shock patients. We analyzed HERV transcriptome with microarray data from whole blood samples of a burn cohort (n=30), a trauma cohort (n=105) and 2 septic shock cohorts (n=28, n=51), and healthy volunteers (HV, n=60). We described expression of the 337 probesets targeting HERV from U133 plus 2.0 microarray in each dataset and then we compared HERVs transcriptional modulation of patients compared to healthy volunteers. Although all 4 cohorts contained very severe patients, the majority of the 337 HERVs was not expressed (around 74% in mean). Each cohort had differentially expressed probesets in patients compared to HV (from 19 to 46). Strikingly, 5 HERVs were in common in all types of severely injured patients, with 4 being up-modulated in patients. We highlighted co-expressed profiles between HERV and nearby gene as well as autonomous HERV expression. We suggest an inflammatory-specific HERV transcriptional response, and importantly, we introduce that the HERVs close to immunity-related genes might have a role on its expression.
Databáze: OpenAIRE
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