Endogenous Retroviruses Transcriptional Modulation After Severe Infection, Trauma and Burn
Autor: | Olivier Tabone, Marine Mommert, Camille Jourdan, Elisabeth Cerrato, Matthieu Legrand, Alain Lepape, Bernard Allaouchiche, Thomas Rimmelé, Alexandre Pachot, Guillaume Monneret, Fabienne Venet, François Mallet, Julien Textoris |
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Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Microarray Neutrophils viruses Endogenous retrovirus Monocytes Cohort Studies Transcriptome 0302 clinical medicine burn host response 2.1 Biological and endogenous factors Immunology and Allergy Aetiology Original Research Statistics Shock Middle Aged Shock Septic Healthy Volunteers trauma Medical Microbiology embryonic structures Cohort Female Burns lcsh:Immunologic diseases. Allergy Adult Physical Injury - Accidents and Adverse Effects Immunology Statistics Nonparametric endogenous retroviruses Sepsis 03 medical and health sciences Clinical Research Immunity Genetics medicine Humans Nonparametric Gene Aged Inflammation Septic Septic shock business.industry Microarray analysis techniques medicine.disease 030104 developmental biology Genetic Loci Wounds and Injuries septic shock lcsh:RC581-607 business transcriptome severe inflammatory injuries 030215 immunology |
Zdroj: | Frontiers in Immunology, Vol 9 (2019) Frontiers in Immunology |
ISSN: | 1664-3224 |
Popis: | Although human endogenous retroviruses (HERVs) expression is a growing subject of interest, no study focused before on specific endogenous retroviruses loci activation in severely injured patients. Yet, HERV reactivation is observed in immunity compromised settings like some cancers and auto-immune diseases. Our objective was to assess the transcriptional modulation of HERVs in burn, trauma and septic shock patients. We analyzed HERV transcriptome with microarray data from whole blood samples of a burn cohort (n=30), a trauma cohort (n=105) and 2 septic shock cohorts (n=28, n=51), and healthy volunteers (HV, n=60). We described expression of the 337 probesets targeting HERV from U133 plus 2.0 microarray in each dataset and then we compared HERVs transcriptional modulation of patients compared to healthy volunteers. Although all 4 cohorts contained very severe patients, the majority of the 337 HERVs was not expressed (around 74% in mean). Each cohort had differentially expressed probesets in patients compared to HV (from 19 to 46). Strikingly, 5 HERVs were in common in all types of severely injured patients, with 4 being up-modulated in patients. We highlighted co-expressed profiles between HERV and nearby gene as well as autonomous HERV expression. We suggest an inflammatory-specific HERV transcriptional response, and importantly, we introduce that the HERVs close to immunity-related genes might have a role on its expression. |
Databáze: | OpenAIRE |
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