Interaction of Human Mesenchymal Stem Cells With Disc Cells
Autor: | Kei Tateno, Seok Woo Kim, Kam W. Leong, Catherine Le Visage, John P. Kostuik, Ann N. Sieber |
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Rok vydání: | 2006 |
Předmět: |
Adult
Type II collagen Bone Marrow Cells Glycosaminoglycan Extracellular matrix medicine Humans Lectins C-Type Orthopedics and Sports Medicine Aggrecans RNA Messenger Fluorescent Antibody Technique Indirect Intervertebral Disc Cells Cultured Glycosaminoglycans Extracellular Matrix Proteins biology Mesenchymal stem cell Mesenchymal Stem Cells Intervertebral disc DNA Anatomy Middle Aged Magnetic Resonance Imaging Coculture Techniques Extracellular Matrix Up-Regulation Cell biology medicine.anatomical_structure Chondroitin Sulfate Proteoglycans Proteoglycan biology.protein Female Neurology (clinical) Bone marrow Stem cell |
Zdroj: | Spine. 31:2036-2042 |
ISSN: | 0362-2436 |
Popis: | Study Design. To evaluate the in vitro interactions between human mesenchymal stem cells (MSCs) and degenerative disc cells. Objectives. To demonstrate the potential of MSCs in regulating the extracellular matrix synthesis of degenerative disc cells. Summary of Background Data. Culture of degenerative disc cells followed by their reinsertion into a disc can retard the degeneration process in an animal model. However, harvesting cells without accelerating degeneration is problematic. Autologous MSCs can be safely harvested from the bone marrow and transplanted into degenerative discs. Methods. Human degenerative nucleus pulposus (NP), anulus fibrosus (AF) cells, and MSCs were cultured as pellets, and coculture pellets were formed by addition of MSCs to disc cells (50:50 ratio). Glycosaminoglycan (GAG) and DNA content were measured. Proteoglycan synthesis was analyzed by RT-PCR and western blot. Type II collagen expression was assessed by immunohistochemistry. Results. Coculture pellets formed by the addition of MSCs to AF cells were superior in size to all other pellets. AF/MSC pellets showed higher experimental GAG content than the predicted values represented by the sum of individual control pellets, with 10.2 versus 5.6 μg/pellet at week 3, respectively. The effect was not observed in the NP/MSC coculture, or when chondrogenic medium was used. Close contact between cells was necessary to obtain this enhancement of GAG content. Proteoglycan and collagen expression in both individual and coculture pellets was confirmed by PCR analysis and western blot. Conclusion. Addition of MSCs to AF cells resulted in an up-regulation of the proteoglycans synthesis. This study provides the rationale for further investigation of the potential of MSC therapy in treating intervertebral disc degeneration. |
Databáze: | OpenAIRE |
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