Comparative binding and uptake of liposomes decorated with mannose oligosaccharides by cells expressing the mannose receptor or DC-SIGN
| Autor: | Virginie Malard, Virginie Cendret, Florence Djedaïni-Pilard, Vincent Moreau, Marc François-Heude, Chantal Pichon, Patrick Midoux, François M. Lemoine, Véronique Mateo, Téo Gallego, Haifei Gao, Cristine Gonçalves |
|---|---|
| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
media_common.quotation_subject
[SDV]Life Sciences [q-bio] Mannose Oligosaccharides Receptors Cell Surface 010402 general chemistry 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Glycolipid Antigen Humans Lectins C-Type Internalization Cells Cultured media_common Liposome Binding Sites biology Molecular Structure 010405 organic chemistry Chemistry Organic Chemistry General Medicine Dendritic Cells 0104 chemical sciences DC-SIGN HEK293 Cells Mannose-Binding Lectins Liposomes Biophysics biology.protein Cell Adhesion Molecules Mannose receptor Intracellular Mannose Receptor |
| Zdroj: | Carbohydrate Research Carbohydrate Research, Elsevier, 2020, 487, pp.107877. ⟨10.1016/j.carres.2019.107877⟩ |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/j.carres.2019.107877⟩ |
| Popis: | International audience; Mannose Receptor (MR) and DC-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) are two mannose-specific targets for antigens carried by liposomes but DC-SIGN is more specific of DCs. Here, DC targeting is addressed by using DPPC/DOPE liposomes decorated with a series of diether lipids with a polar head of either a mannose (Man), tri-antenna of α-d-mannopyranoside (Tri-Man), [Manα1-3(Manα1-6)Man] (Man-tri), pseudo-Man4 (PMan4) or pseudo-Man5 (PMan5). Liposomes decorated with Man-Tri show the highest binding and internalization in cells expressing DC-SIGN and in human monocytes-derived DCs. Conversely, cells expressing MR bind and take up Tri-Man liposomes 3-fold higher than Man-tri liposomes. Comparatively, liposomes decorated with PMan4 and PMan5 do not show any advantages. Overall, the results indicate that liposomes decorated with Man-tri residues are more selective toward DCs than those with Tri-Man thanks to better recognition by DC-SIGN. |
| Databáze: | OpenAIRE |
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