Prevalência de infecção por Cryptosporidium em gatos domiciliados em uma área urbana no Brasil
| Autor: | Marcelo Vasconcelos Meireles, Lucas Vinicius Shigaki de Matos, Marcel G. Marques, Giovanni Widmer, Ana Elisa G. W. Marques, Luiz da Silveira Neto, Mariele Fernanda da Cruz Panegossi, Valéria Marçal Felix de Lima, Katia Denise Saraiva Bresciani, Sandra Valéria Inácio |
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| Přispěvatelé: | UFT, Tufts University, Universidade Estadual Paulista (Unesp), UNIFIO, Ipanema Indústria de Produtos Veterinários, Centro Universitário Max Planck |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
Diagnostic methods CATS biology Cryptosporidium infection Capture elisa Epidemiology Cryptosporidiosis Cryptosporidium Sampling error biology.organism_classification medicine.disease Feline Genetic similarity Cryptosporidium felis medicine Prevalence Public Health General Agricultural and Biological Sciences |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-25T10:34:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 We investigated the occurrence of Cryptosporidium oocysts shedding by domestic cats in an urban setting. The calculation of minimum sample size was based on an estimated prevalence of 10%, 5% absolute sampling error and a 5% significance level, resulting in 138 cats. A total of 612 owners of 2,290 cats had to be contacted for achieving the minimal sample size. In the end, only 55 owners accepted to participate in this investigation. Stool samples collected from 138 dogs were examined by microscopy using modified Kinyoun acid-fast staining, capture ELISA and nested-PCR followed by sequencing. Samples were considered positive when Cryptosporidium were detected by at least two diagnostic methods. Thirteen samples were positive (9.4%; 95% CI: 4.5 - 14.3). Cryptosporidium amplicons from seven out of the 13 samples were successfully sequenced and shared 99% genetic similarity to Cryptosporidium felis, GenBank access AF112575.1 was found. We concluded that Cryptosporidium infection is common in domestic cats from urban area and veterinary practitioners should guide cat owners to adopt preventive measures against the parasite to reduce the chance of infection in cats and householders. Engenharia de Bioprocessos e Biotecnologia Universidade Federal do Tocantins UFT Cummings School of Veterinary Medicine Tufts University Universidade Estadual Paulista UNESP UNESP Centro Universitário das Faculdades Integradas de Ourinhos UNIFIO Pós-Graduaçãoem Ciência Animal UNESP Ipanema Indústria de Produtos Veterinários Curso de Medicina Veterinária Centro Universitário Max Planck Universidade Estadual Paulista UNESP UNESP Pós-Graduaçãoem Ciência Animal UNESP |
| Databáze: | OpenAIRE |
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