Development of an enzyme-linked immunosorbent assay for the serodiagnosis of several clinical forms of sporotrichosis
| Autor: | Eliane Tatsch Neves, Marise Mattos, Leila M. Lopes-Bezerra, Clara Paz Dias, M. P. Oliveira Neto, Márcia dos Santos Lazéra, Andréa Reis Bernardes-Engemann, Carla V. Loureiro y Penha, M. C. Gutierrez, R. C Orofino Costa, B. P Miguens, Bernardo Acácio Santini Pereira, A. Schubach |
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| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Antigens Fungal Enzyme-Linked Immunosorbent Assay HIV Infections Sensitivity and Specificity Serology Antigen Cell Wall Humans Medicine Sporothrix schenckii Antibodies Fungal Plant Proteins AIDS-Related Opportunistic Infections biology Sporotrichosis business.industry Sporothrix Glycopeptides General Medicine biology.organism_classification medicine.disease Titer Infectious Diseases Immunology biology.protein Antibody Differential diagnosis business |
| Zdroj: | Medical Mycology. 43:487-493 |
| ISSN: | 1460-2709 1369-3786 |
| DOI: | 10.1080/13693780400019909 |
| Popis: | We performed a serological study with sera from 92 patients with confirmed sporotrichosis registered between 1999 and 2004 in two hospitals in Rio de Janeiro State, Brazil. The clinical presentation of sporotrichosis was distributed as follows: lymphocutaneous, 67%; fixed cutaneous, 23%; disseminated cutaneous, 8%; and extracutaneous, 2%. Sera were assayed by ELISA against a cell wall antigen of Sporothrix schenckii, SsCBF, that we have previously described. The cross-reactivity was determined with 77 heterologous sera. The serological test showed a sensitivity of 90% and a global efficiency of 86%. A group of 55 patients with several clinical presentations of sporotrichosis was clinically and serologically followed-up for at least 6 months. We observed by ELISA data a decrease in the antibody serum titers which correlated with the progress in healing. An HIV-positive patient with meningeal sporotrichosis was serologically followed-up for over 2 years. Serum and cerebrospinal fluid specimens were examined and significant antibodies levels against the antigen SsCBF were detected. Our results strongly suggest that this serological test is valuable for the differential diagnosis and follow-up of all clinical forms of sporotrichosis. |
| Databáze: | OpenAIRE |
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