Endogenous glutamatergic inputs to the Parabrachial Nucleus/ Kölliker-Fuse Complex determine respiratory rate
| Autor: | Jennifer J. Callison, Edward J. Zuperku, Angela Navarrete-Opazo, Barbara Palkovic, Denise R. Cook-Snyder, Nicole McCarthy, Justin R. Miller, Astrid G. Stucke, Eckehard A. E. Stuth |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Respiratory rate Microinjections Physiology Glutamic Acid Article 03 medical and health sciences chemistry.chemical_compound Glutamatergic 0302 clinical medicine Respiratory Rate In vivo Internal medicine Quinoxalines medicine Excitatory Amino Acid Agonists Animals Kolliker-Fuse Nucleus Receptor alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Parabrachial Nucleus General Neuroscience Hypoxia (medical) Respiratory Center medicine.anatomical_structure 030228 respiratory system chemistry Cardiology NBQX Female Rabbits medicine.symptom Nucleus Excitatory Amino Acid Antagonists 030217 neurology & neurosurgery |
| Zdroj: | Respir Physiol Neurobiol |
| Popis: | The Kolliker-Fuse Nucleus (KF) has been widely investigated for its contribution to "inspiratory off-switch" while more recent studies showed that activation of the Parabrachial Nucleus (PBN) shortened expiratory duration. This study used an adult, in vivo, decerebrate rabbit model to delineate the contribution of each site to inspiratory and expiratory duration through sequential block of glutamatergic excitation with the receptor antagonists 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX) and d(-)-2-amino-5-phosphonopentanoic acid (AP5). Glutamatergic disfacilitation caused large increases in inspiratory and expiratory duration and minor decrease in peak phrenic activity (PPA). Hypoxia only partially reversed respiratory rate depression but PPA was increased to >200 % of control. The contribution of PBN activity to inspiratory and expiratory duration was equal while block of the KF affected inspiratory duration more than expiratory. We conclude that in the in vivo preparation respiratory rate greatly depends on PBN/KF activity, which contributes to the "inspiratory on- "and "off-switch", but is of minor importance for the magnitude of phrenic motor output. |
| Databáze: | OpenAIRE |
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