Notch promotes survival of neural precursor cells via mechanisms distinct from those regulating neurogenesis
| Autor: | Masato Nakafuku, Koji Oishi, Keisuke Kuida, Sachiko Kamakura, Yukiko Gotoh, Norihisa Masuyama, Takeshi Yoshimatsu, Yuko Isazawa |
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| Rok vydání: | 2004 |
| Předmět: |
Programmed cell death
Cell Survival Green Fluorescent Proteins Immunoblotting Notch signaling pathway Succinimides Biology Mice RNA interference Precursor cell Animals Luciferases Molecular Biology Cells Cultured Fluorescent Dyes Mice Knockout Neurons Gene knockdown Receptors Notch Quinolinium Compounds Stem Cells Neurogenesis Antibodies Monoclonal Gene Expression Regulation Developmental Membrane Proteins Cell Biology Immunohistochemistry Embryonic stem cell Protein Structure Tertiary Up-Regulation Cell biology Retroviridae Microscopy Fluorescence Notch proteins NIH 3T3 Cells RNA Interference Gene Deletion Developmental Biology |
| Zdroj: | Developmental Biology. 276:172-184 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2004.08.039 |
| Popis: | During development of the mammalian brain, many neural precursor cells (NPCs) undergo apoptosis. The regulation of such cell death, however, is poorly understood. We now show that the survival of mouse embryonic NPCs in vitro was increased by culture at a high cell density and that this effect was attributable to activation of Notch signaling. Expression of an active form of Notch1 thus markedly promoted NPC survival. Hes proteins, key effectors of Notch signaling in inhibition of neurogenesis, were not sufficient for the survival-promoting effect of Notch1. This effect of Notch1 required a region of the protein containing the RAM domain and was accompanied by up-regulation of the anti-apoptotic proteins Bcl-2 and Mcl-1. Moreover, knockdown of these proteins by RNA interference resulted in blockade of the Notch1-induced survival. These results reveal a new function of Notch, the promotion of NPC survival. |
| Databáze: | OpenAIRE |
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