Transcriptional responses of rat skeletal muscle following hypoxia-reoxygenation and near ischaemia-reperfusion
| Autor: | Andrew D. Shaw, Bernhard Riedel, M. T. Williams, Natarajan Aravindan |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Pathology Transcription Genetic Physiology medicine.medical_treatment Ischemia Hindlimb Biology Rats Sprague-Dawley Internal medicine Gene expression medicine Animals Hypoxia Muscle Skeletal Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Kinase Microarray analysis techniques Growth factor Skeletal muscle Hypoxia (medical) medicine.disease Rats Up-Regulation Oxygen medicine.anatomical_structure Endocrinology Gene Expression Regulation medicine.symptom |
| Zdroj: | Acta Physiologica Scandinavica. 183:367-377 |
| ISSN: | 1365-201X 0001-6772 |
| DOI: | 10.1111/j.1365-201x.2005.01413.x |
| Popis: | Aim: The effect of ischaemia/reperfusion or hypoxia/reoxygenation on gene expression has not been extensively studied. We hypothesized that in skeletal muscle, tissue hypoxia of similar magnitude but induced by different mechanisms would lead to different transcriptional responses. Methods: Muscle gene transcription was assessed using microarray analysis and reverse transcriptase polymerase chain reaction in 18 rats exposed to regional hind limb near ischaemia/reperfusion (n = 6), hypoxia/reoxygenation (n = 6) or sham operation (n = 6). Hypoxic burden was measured by the area under the PtO2–time curve. Results: PtO2 was reduced in both the near ischaemia/reperfusion and hypoxia/reoxygenation groups. Although the hypoxic burden was similar, the genomic response was different for each condition. Near ischaemia/reperfusion had a greater effect on gene expression than hypoxia/reoxygenation. Using stringent criteria for changes in gene expression (i.e. more than or equal to twofold change vs. control) unique patterns of gene expression could be identified suggesting individualized transcriptional responses to each of these injuries. Several genes, including insulin-like growth factor 1 (IGF-1) and cyclin-dependent kinase inhibitor (p27Kip1) were induced by both injury types and these may have potential clinical application as markers of tissue damage. In contrast, no single gene was downregulated by both injury conditions. Conclusions: The mechanism of skeletal muscle hypoxia has a profound effect on its subsequent transcriptional response. We identified several potential candidates as markers of skeletal muscle ischaemic damage. |
| Databáze: | OpenAIRE |
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