Nov/CCN3 regulates long-term repopulating activity of murine hematopoietic stem cells via integrin αvβ3
| Autor: | Masayuki Yamato, Jun Ishihara, Koji Eto, Terumasa Umemoto, Brian G. Petrich, Yoshiko Shiratsuchi, Toshio Kitamura, Teruo Okano, Satoshi Takaki, Hiromitsu Nakauchi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cellular differentiation
Immunology Integrin Gene Expression Article Interferon-gamma Mice Nephroblastoma Overexpressed Protein medicine Animals 1102 Cardiorespiratory Medicine and Haematology Integrin alphaVbeta3 biology Matricellular protein Hematopoietic Stem Cell Transplantation Hematopoietic stem cell hemic and immune systems Cell Differentiation Hematology Hematopoietic Stem Cells Haematopoiesis medicine.anatomical_structure STAT1 Transcription Factor Integrin alpha M Thrombopoietin Cancer research biology.protein Stem cell Signal Transduction |
| Popis: | Throughout life, hematopoietic stem cells (HSCs) sustain the blood cell supply through their capacities for self-renewal and multilineage differentiation. These processes are regulated within a specialized microenvironment termed the ‘niche’. Here, we show a novel mechanism for regulating HSC function that is mediated by nephroblastoma overexpressed (Nov/CCN3), a matricellular protein member of the CCN family. We found that Nov contributes to the maintenance of long-term repopulating (LTR) activity through association with integrin αvβ3 on HSCs. The resultant β3 integrin outside-in signaling is dependent on thrombopoietin (TPO), a crucial cytokine involved in HSC maintenance. TPO was required for Nov binding to integrin αvβ3, and stimulated Nov expression in HSCs. However, in the presence of IFNγ, a cytokine known to impair HSC function, not only was TPO-induced expression of Nov suppressed, but the LTR activity was conversely impaired by TPO-mediated ligation of integrin αvβ3 with exogenous ligands, including Nov, as well. Thus, Nov/integrin αvβ3-mediated maintenance of HSCs appears to be modulated by simultaneous stimulation by other cytokines. Our finding suggests that this system contributes to the regulation of HSCs within the bone marrow niche. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|