A Modular Millifluidic Homeostatic Imaging Plate for Imaging of Larval Zebrafish
| Autor: | Timo Friedrich, Alon M. Douek, Jan Kaslin, Celia Vandestadt, Donald Wlodkowic |
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| Rok vydání: | 2019 |
| Předmět: |
Biomedical instrumentation
Immobilization 03 medical and health sciences 0302 clinical medicine Lab-On-A-Chip Devices Zebrafish larvae Animals Zebrafish 030304 developmental biology Microscopy 0303 health sciences biology business.industry Regeneration (biology) fungi Microfluidic Analytical Techniques Modular design biology.organism_classification Printing Three-Dimensional Animal Science and Zoology business 030217 neurology & neurosurgery Preclinical imaging Developmental Biology Biomedical engineering |
| Zdroj: | Zebrafish. 16:37-46 |
| ISSN: | 1557-8542 1545-8547 |
| DOI: | 10.1089/zeb.2018.1684 |
| Popis: | Zebrafish larvae are suitable in vivo models for toxicological and pharmacological screens due to their transparency, small size, ex utero development, and genetic and physiological similarity to humans. Using modern imaging techniques, cells and tissues can be dynamically visualized over several days in multiple zebrafish larvae. However, precise specimen immobilization and maintenance of homeostatic conditions remain a challenge for longitudinal studies. A highly customizable mounting configuration with inbuilt means of controlling temperature and media flow would therefore be a valuable tool to facilitate long-term imaging of a large number of specimens. Using three-dimensional printing, we have developed a millifluidic, modular homeostatic imaging plate (HIP), which consists of a customizable sample insert and a temperature-controlled incubation chamber that is continuously perfused, providing an ideal environment for long-term experiments where homeostatic conditions are desired. The HIP is cheap to produce, has a standard microtiter well plate format, and can be fitted to most microscopes. We used the device to image dynamic regeneration of spinal cord neurons. The flexibility and adaptability of the HIP facilitate long-term in vivo imaging of many samples, and can be easily adapted to suit a broad range of specimens. |
| Databáze: | OpenAIRE |
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