Olfactory Reception of Host Alarm Pheromone Component by the Odorant-Binding Proteins in the Samurai Wasp, Trissolcus japonicus (Hymenoptera: Scelionidae)
| Autor: | Jinping Zhang, Yongzhi Zhong, Rui Tang, K. B. Rebijith, Fengqi Li, Guohua Chen, Feng Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Genetics
lcsh:QP1-981 biology Physiology Odorant binding Host (biology) fungi Hymenoptera biology.organism_classification brown marmorated stink bug insect olfaction lcsh:Physiology Parasitoid (E)-2-decenal Physiology (medical) egg parasitoid Pheromone Heterologous expression Brown marmorated stink bug transcriptome Scelionidae |
| Zdroj: | Frontiers in Physiology, Vol 11 (2020) |
| ISSN: | 1664-042X |
| DOI: | 10.3389/fphys.2020.01058 |
| Popis: | The samurai wasp, Trissolcus japonicus, is the predominant egg parasitoid of the brown marmorated stink bug, Halyomorpha halys, in its native range in China. (E)-2-Decenal is a major component of the alarm pheromone of H. halys, an important invasive insect pest with significant economic importance. T. japonicus can be strongly repelled by (E)-2-decenal, and thus its host location efficiency would be reduced in the field. Better understanding on the molecular basis of olfactory reception of this host alarm pheromone component by T. japonicus may provide opportunities to develop novel approaches to enhance biological control efficacy of the parasitoid against H. halys. We identified six Odorant Binding Proteins (OBPs) from T. japonicus by transcriptome sequencing, within which three classical OBPs were expressed in a heterologous expression system with E. coli, harvested, and then challenged with (E)-2-decenal in binding assay experiments. TjapOBP2 showed the highest binding ability to (E)-2-decenal, compared to TjapOBP1 and TjapOBP3. Our results unambiguously suggest that TjapOBP2 would play an important role in discriminating (E)-2-decenal and could be a possible target for further intervention in the parasitoid–host system. |
| Databáze: | OpenAIRE |
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