Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
| Autor: | Klaudia dos Santos Gonçalves Jorge, Ana Luiza Alves Rosa Osório, Clarice Queico Fugimura Leite, Cristina Pires de Araújo, Karina Andrade de Prince |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Mato Grosso do Sul (UFMS) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
nontuberculous mycobacteria DNA Bacterial Bacilli Tuberculosis lcsh:Arctic medicine. Tropical medicine Meat Genotype lcsh:RC955-962 Restriction Mapping lcsh:QR1-502 Micobactérias não Tuberculosas Matadouros Polymerase Chain Reaction lcsh:Microbiology Mycobacterium Bovis Bovinos law.invention Microbiology fluids and secretions law medicine Animals Polymerase chain reaction Mycobacterium bovis biology Nontuberculous Mycobacteria biology.organism_classification medicine.disease bacterial infections and mycoses Staining Bacterial Typing Techniques post-mortem inspection Restriction enzyme PCR- restriction enzyme pattern analysis Nontuberculous mycobacteria Cattle Tuberculosis Bovine Brazil Abattoirs Reação em Cadeia da Polimerase |
| Zdroj: | Repositório Institucional da UFMS Universidade Federal de Mato Grosso do Sul (UFMS) instacron:UFMS Memórias do Instituto Oswaldo Cruz, Volume: 100, Issue: 7, Pages: 749-752, Published: NOV 2005 SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Memórias do Instituto Oswaldo Cruz., Vol 100, Iss 7, Pp 749-752 (2005) |
| ISSN: | 0074-0276 |
| Popis: | Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:45:33Z No. of bitstreams: 1 S0074-02762005000700013.pdf: 592978 bytes, checksum: b218d4ce4f9773ae31667422408a25df (MD5) Made available in DSpace on 2013-08-22T18:45:33Z (GMT). No. of bitstreams: 1 S0074-02762005000700013.pdf: 592978 bytes, checksum: b218d4ce4f9773ae31667422408a25df (MD5) Previous issue date: 2005-11-01 Made available in DSpace on 2013-09-30T18:07:32Z (GMT). No. of bitstreams: 2 S0074-02762005000700013.pdf: 592978 bytes, checksum: b218d4ce4f9773ae31667422408a25df (MD5) S0074-02762005000700013.pdf.txt: 15308 bytes, checksum: b283ba4e81a075da9e840409b2ac11a5 (MD5) Previous issue date: 2005-11-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:25:21Z No. of bitstreams: 2 S0074-02762005000700013.pdf: 592978 bytes, checksum: b218d4ce4f9773ae31667422408a25df (MD5) S0074-02762005000700013.pdf.txt: 15308 bytes, checksum: b283ba4e81a075da9e840409b2ac11a5 (MD5) Made available in DSpace on 2014-05-20T13:25:21Z (GMT). No. of bitstreams: 2 S0074-02762005000700013.pdf: 592978 bytes, checksum: b218d4ce4f9773ae31667422408a25df (MD5) S0074-02762005000700013.pdf.txt: 15308 bytes, checksum: b283ba4e81a075da9e840409b2ac11a5 (MD5) Previous issue date: 2005-11-01 The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul. Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas Universidade Federal de Mato Grosso do Sul Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Farmacêuticas |
| Databáze: | OpenAIRE |
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