Mesenchymal stem cells of Systemic Sclerosis patients, derived from different sources, show a profibrotic microRNA profiling
| Autor: | Edoardo Alesse, Noemi Panzera, Giuliana Guggino, Francesco Ciccia, Piero Ruscitti, Alessandra Tessitore, Roberto Giacomelli, Francesco Carubbi, Vasiliki Liakouli, Antinisca Di Marco, Valentina Mastroiaco, Onorina Berardicurti, Paola Cipriani, Paola Di Benedetto, Andrea Bianchi |
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| Přispěvatelé: | Di Benedetto, P., Panzera, N., Cipriani, P., Mastroiaco, V., Tessitore, A., Liakouli, V., Ruscitti, P., Berardicurti, O., Carubbi, F., Guggino, G., Bianchi, A., Di Marco, A., Ciccia, F., Alesse, E., Giacomelli, R., Di Benedetto P., Panzera N., Cipriani P., Mastroiaco V., Tessitore A., Liakouli V., Ruscitti P., Berardicurti O., Carubbi F., Guggino G., Bianchi A., Di Marco A., Ciccia F., Alesse E., Giacomelli R. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Therapeutic gene modulation Autoimmune diseases Cellular differentiation Gene regulatory network lcsh:Medicine Bone Marrow Cells Biology Regenerative medicine Article 03 medical and health sciences 0302 clinical medicine microRNA medicine Humans Gene Regulatory Networks lcsh:Science Cells Cultured Systemic Sclerosi Cell Proliferation Regulation of gene expression Scleroderma Systemic Multidisciplinary Sequence Analysis RNA Gene Expression Profiling Mesenchymal stem cell lcsh:R Cell Differentiation Mesenchymal Stem Cells Settore MED/16 - Reumatologia MicroRNAs 030104 developmental biology medicine.anatomical_structure Adipose Tissue Gene Expression Regulation Cancer research Systemic sclerosis Female lcsh:Q Bone marrow 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Systemic Sclerosis (SSc) is a disease with limited therapeutic possibilities. Mesenchymal stem cells (MSCs)-therapy could be a promising therapeutic option, however the ideal MSCs source has not yet been found. To address this problem, we perform comparison between bone marrow (BM)-MSCs and adipose (A)-MSCs, by the miRs expression profile, to identify the gene modulation in these two MSCs source. MicroRNAs (miRs) are RNAs sequences, regulating gene expression and MSCs, derived from different tissues, may differently respond to the SSc microenvironment. The miRs array was used for the miRs profiling and by DIANA-mirPath tool we identified the biological functions of the dysregulated miRs. In SSc-BM-MSCs, 6 miRs were significantly down-regulated and 4 miRs up-regulated. In SSc-A-MSCs, 11 miRs were significantly down-regulated and 3 miRs up-regulated. Interestingly, in both the sources, the involved pathways included the senescence mechanisms and the pro-fibrotic behaviour. Furthermore, both the MSCs sources showed potential compensatory ability. A deeper knowledge of this miRs signature might give more information about some pathogenic steps of the disease and in the same time clarify the possible therapeutic role of autologous MSCs in the regenerative therapy in SSc. |
| Databáze: | OpenAIRE |
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