Transient disruption of mouse home cage activities and assessment of orexin immunoreactivity following concussive- or blast-induced brain injury
| Autor: | Thanhlong Tran, Yeonho Kim, Laura B. Tucker, Patricia A. Vu, Joseph T. McCabe, Eileen H. McNamara, Jiong Liu, Amanda H. Fu |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Traumatic brain injury Motor Activity Open field Random Allocation 03 medical and health sciences 0302 clinical medicine Blast Injuries Internal medicine Brain Injuries Traumatic Concussion medicine Animals Molecular Biology Neurons Orexins Behavior Animal business.industry General Neuroscience Brain medicine.disease Housing Animal Orexin Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology Shock (circulatory) Neurology (clinical) medicine.symptom business Hypoactivity human activities 030217 neurology & neurosurgery Immunostaining Ingestive behaviors Developmental Biology |
| Zdroj: | Brain Research. 1700:138-151 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2018.08.034 |
| Popis: | The employment of explosive weaponry in modern warfare exposes populations to shock wave-induced and impact-related brain injuries. Among the most common clinical complaints resulting from traumatic brain injury (TBI) are sleep-wake disturbances. The current study assessed the acute effects of mild concussive brain injury (CBI) and mild blast wave-induced brain injury (BTBI) on mouse behavior and orexin-A expression. Male C57BL/6J mice were exposed to CBI, BTBI, or sham procedures. Injured animals and their shams were further divided into the following subgroups: 24-h survival in standard group (SG) housing, 72-h survival in SG housing, and 72-h survival in Any-Maze cages (AMc). AMc enabled continuous monitoring of home cage activities. BTBI caused significant but transient decreases in wheel running and ingestive behaviors 24 h post-injury (PI), while CBI transiently decreased running and water intake. BTBI resulted in general hypoactivity in the open field (OF) at both PI time points for SG-housed animals. In contrast, CBI did not cause hypoactivity. Mice subjected to CBI traveled more in the center of the OF at both time points PI, suggesting that CBI caused reduced anxiety in mice. Increased activity in the center of the OF was also seen at 24 h PI after BTBI. CBI treatment caused increased CD11b immunostaining. However, neither injury was accompanied by an alteration in the number of orexin-A hypothalamic neurons. Taken together, shock wave exposure and concussive injury transiently reduced mouse activities, but some differences between the two injuries were seen. |
| Databáze: | OpenAIRE |
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