p53 Mutation and overexpression in hepatocellular carcinoma and dysplastic nodules in the liver
| Autor: | Yun Kyung Kang, Yang Il Kim, Hyun Ok Kim, Chong Jai Kim, Gyeong Hoon Kang, Woo Ho Kim |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Liver Cirrhosis
Male Carcinoma Hepatocellular Biology Polymerase Chain Reaction Pathology and Forensic Medicine Immunoenzyme Techniques Exon Gene expression medicine Carcinoma Humans Point Mutation Missense mutation Molecular Biology Polymorphism Single-Stranded Conformational Aged DNA Primers Point mutation Liver Neoplasms Antibodies Monoclonal Cell Biology General Medicine Middle Aged HCCS Genes p53 medicine.disease Hepatocellular carcinoma Cancer research Immunohistochemistry Electrophoresis Polyacrylamide Gel Female Tumor Suppressor Protein p53 |
| Zdroj: | Virchows Archiv. 432:27-32 |
| ISSN: | 1432-2307 0945-6317 |
| DOI: | 10.1007/s004280050130 |
| Popis: | In hepatocarcinogenesis, both de novo and multistep pathways have been suggested and in the latter a dysplastic nodule is the proposed precancerous lesion. In this study, we tried to ascertain whether or not the p53 gene is altered in low-grade/high-grade dysplastic nodules (LDN/HDN) and to determine the role of p53 alteration in multistep hepatocarcinogenesis. Eight hepatocellular carcinomas (HCCs), 9 HDNs, 17 LDNs and 25 cirrhotic nodules (LCs) were examined by polymerase chain reaction-single strand conformation polymorphism/direct sequencing and immunohistochemical staining for p53. Four of the 8 HCCs (50%) revealed p53 overexpression and 2 (25%) had missense mutations. Four of the 9 HDNs (44%) showed weak and/or focal p53 overexpression but none had mutation in the exons examined. Neither p53 overexpression nor mutation was found in 17 LDNs and 25 LCs. These results suggest that p53 mutation might be an unusual event in precancerous lesions of multistep hepatocarcinogenesis (DN-HCC sequence) and may play a less crucial part than in colorectal carcinogenesis. |
| Databáze: | OpenAIRE |
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