Radiosensitization by bromodeoxyuridine and hyperthermia: analysis of linear and quadratic parameters of radiation survival curves of two human tumor cell lines
| Autor: | Nicolaas A. P. Franken, Hans M. Rodermond, Jaap Haveman, Martijn A. T. Veltmaat, G. W. Barendsen, Chris van Bree |
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| Přispěvatelé: | Faculteit der Geneeskunde, Other departments |
| Rok vydání: | 2001 |
| Předmět: |
Hyperthermia
Radiation-Sensitizing Agents Hot Temperature Health Toxicology and Mutagenesis medicine.medical_treatment Cell Biology Radiation Tolerance Ionizing radiation chemistry.chemical_compound Tumor Cells Cultured medicine Humans Radiology Nuclear Medicine and imaging Beta (finance) Clonogenic assay Sensitization Radiation Cell Death medicine.disease Radiation therapy medicine.anatomical_structure Bromodeoxyuridine chemistry Immunology Cancer research |
| Zdroj: | Journal of Radiation Research, 42, 179-190. Japan Radiation Research Society Journal of radiation research, 42(2), 179-190. Japan Radiation Research Society |
| ISSN: | 1349-9157 0449-3060 |
| DOI: | 10.1269/jrr.42.179 |
| Popis: | Sensitization by bromodeoxyuridine (BrdUrd) and hyperthermia (HT) on cell reproductive death induced by ionizing radiation was analyzed using the linear-quadratic [S(D)/S(0)=exp(-(alphaD + betaD2)]] model. Plateau-phase human lung tumor cells (SW-1573) and human colorectal carcinonoma cells (RKO) were treated with BrdUrd, radiation and HT. LQ-analysis was performed at iso-incubation dose and at iso-incorporation level of BrdUrd. and at iso-HT doses and iso-survival levels after HT. Clonogenic assays were performed 24 h after treatment to allow repair of potentially lethal damage (PLD). In SW cells BrdUrd. HT or the combination significantly increased the alpha-parameter (factor 2.0-5.7), without altering the beta-parameter. In RKO cells sensitization with BrdUrd increased both a (factor 1.4) and beta (factor 1.3) while HT only influenced beta (factor 2.1-4.0). The combination did not further increase the a and beta. The results indicate that BrdUrd has its main effect on the parameter alpha, dominant at clinically relevant radiation doses but that HT can affect both a and beta. The addition of BrdUrd and HT provides a method to enhance the efficacy of radiotherapy. |
| Databáze: | OpenAIRE |
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