Normal and Deficient Neutrophils Can Cooperate to Damage Aspergillus fumigatus Hyphae
| Autor: | John H. Rex, D A Melnick, John E. Bennett, Harry L. Malech, John I. Gallin |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Hypha
Neutrophils Population Dose-Response Relationship Immunologic Granulomatous Disease Chronic Microbiology Aspergillus fumigatus Superoxide dismutase Chronic granulomatous disease medicine Humans Immunology and Allergy education Cells Cultured Peroxidase education.field_of_study biology Chemistry fungi medicine.disease biology.organism_classification Respiratory burst Microscopy Electron Infectious Diseases Catalase Myeloperoxidase biology.protein Oxidation-Reduction |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/162.2.523 |
| Popis: | Using a metabolic test of hyphal viability, the interaction between neutrophils and Aspergillus hyphae was investigated over a broad range of hyphae-to-neutrophil ratios. Normal neutrophils were found to damage hyphae whereas neutrophils from patients with both chronic granulomatous disease (CGD) and myeloperoxidase (MPO) deficiency did not. Further, both azide and catalase + superoxide dismutase inhibited the ability of normal neutrophils to damage hyphae, suggesting that this damage is mediated by products of the respiratory burst and by the MPO-halide system. Also, mixtures of small numbers of normal neutrophils with larger numbers of CGD neutrophils (range, 1:5 to 1:15) damaged hyphae more efficiently than either population of cells alone. Further, mixtures of CGD and MPO-deficient neutrophils, neither of which alone could efficiently damage hyphae, were able to damage the hyphae almost as well as a comparable number of normal neutrophils. These data demonstrate that intact neutrophils can cooperate to synergistically damage Aspergillus hyphae, possibly by extracellular mixing of hydrogen peroxide and MPO. |
| Databáze: | OpenAIRE |
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