A convenient method for preparation of biologically active recombinant CHH of the kuruma prawn, Marsupenaeus japonicus, using the bacterial expression system
| Autor: | Shinji Nagata, Chiaki Nagai, Tsuyoshi Ohira, Hideaki Asazuma, Hiromichi Nagasawa |
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| Rok vydání: | 2009 |
| Předmět: |
Invertebrate Hormones
Physiology Molecular Sequence Data Nerve Tissue Proteins Marsupenaeus Biochemistry Arthropod Proteins law.invention Cellular and Molecular Neuroscience chemistry.chemical_compound Endocrinology Penaeidae law Hemolymph Gene expression Animals Amino Acid Sequence Protein secondary structure Chromatography High Pressure Liquid HEPES biology Circular Dichroism Biological activity biology.organism_classification Recombinant Proteins chemistry Hyperglycemia Prawn Recombinant DNA Biological Assay Electrophoresis Polyacrylamide Gel |
| Zdroj: | Peptides. 30:507-517 |
| ISSN: | 0196-9781 |
| Popis: | Crustacean hyperglycemic hormone (CHH) not only plays an important role in the modulation of hemolymph glucose level but also functions in other biological events including molting, reproduction and stress response. Of the six CHHs characterized in Marsupenaeus japonicus, an expression system for recombinant Pej-SGP-VII (rPej-SGP-VII-amide) has not yet been established. Here, we established a procedure using a Nus-tag for solubilization, thereby soluble and biologically active rPej-SGP-VII-amide could successfully be obtained by a simpler procedure than previous ones used for producing other recombinant Pej-SGPs (Pej-SGP-I, III and IV). It was found that rPej-SGP-VII-amide thus obtained had the correct arrangement of intramolecular disulfide bonds and helix-rich secondary structure. The established expression system for rPej-SGP-VII-amide may be applicable for the preparation of other recombinant CHHs. |
| Databáze: | OpenAIRE |
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