A Microfluidic Human Model of Blood–Brain Barrier Employing Primary Human Astrocytes
| Autor: | Lorenzo Bello, Marco Rasile, Davide Pozzi, Eliana Lauranzano, Ruggero Pardi, Lorena Passoni, Michela Matteoli, Raffaella Molteni, Marco Pizzocri, Elena Campo, Ana Ruiz‐Moreno |
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| Přispěvatelé: | Lauranzano, E., Campo, E., Rasile, M., Molteni, R., Pizzocri, M., Passoni, L., Bello, L., Pozzi, D., Pardi, R., Matteoli, M., Ruiz-Moreno, A. |
| Rok vydání: | 2019 |
| Předmět: |
Primary Cell Culture
Models Neurological Central nervous system microfluidics T cells Models Cardiovascular Biomedical Engineering Microfluidic Analytical Techniques Biology blood–brain barrier Blood–brain barrier cytokines General Biochemistry Genetics and Molecular Biology Biomaterials medicine.anatomical_structure Drug development Blood-Brain Barrier Astrocytes Parenchyma medicine Humans Neuroscience Homeostasis |
| Zdroj: | Advanced Biosystems. :1800335 |
| ISSN: | 2366-7478 |
| DOI: | 10.1002/adbi.201800335 |
| Popis: | The neurovascular unit (NVU) is the most important biological barrier between vascular districts and central nervous system (CNS) parenchyma, which maintains brain homeostasis, protects the CNS from pathogens penetration, and mediates neuroimmune communication. T lymphocytes migration across the blood-brain barrier is heavily affected in different brain diseases, representing a major target for novel drug development. In vitro models of NVU could represent a primary tool to investigate the molecular events occurring at this interface. To move toward the establishment of personalized therapies, a patient-related NVU-model is set, incorporating human primary astrocytes integrated into a microfluidic platform. The model is morphologically and functionally characterized, proving to be an advantageous tool to investigate human T lymphocytes transmigration and thus the efficacy of potential novel drugs affecting this process. |
| Databáze: | OpenAIRE |
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