Endless variety for bovine virus diarrhea viruses: new members of a novel subgroup into Pestivirus A from Turkey
Autor: | Tuba Çiğdem Oğuzoğlu, Fırat Doğan, Selda Duran-Yelken, Bahattin Taylan Koç, Nüvit Coşkun |
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Rok vydání: | 2019 |
Předmět: |
Untranslated region
Turkey 040301 veterinary sciences animal diseases viruses Enzyme-Linked Immunosorbent Assay 0403 veterinary science Food Animals Antigen Animals Gene Phylogeny Polymerase biology Sequence Analysis RNA Diarrhea Virus 1 Bovine Viral Pestivirus 0402 animal and dairy science 04 agricultural and veterinary sciences Amplicon biology.organism_classification 040201 dairy & animal science Virology Reverse transcriptase Herd biology.protein Bovine Virus Diarrhea-Mucosal Disease Cattle Female Animal Science and Zoology |
Zdroj: | Tropical Animal Health and Production. 51:1083-1087 |
ISSN: | 1573-7438 0049-4747 |
Popis: | As ubiquitous pathogens, bovine virus diarrhea viruses (BVDVs) in cattle have been reported several times in Turkey. Over time, the frequency and importance of this infection has increased for the livestock industries. A total of 1291 animals were sampled from a dairy herd in Turkey suspected of BVDV clinical signs, for instance, reproductive failures (abortion, congenital malformations in calves, repeat breeding, etc.) and interdigital phlegmon in adult animals. Reverse transcription polymerase chain reactions (RT-PCRs) were made by using targeted 5′ untranslated region (UTR), Npro, E2, and NS2-3 pestiviral gene region primers for antigen ELISA-positive samples (n = 20). The obtained amplicons were sequenced. Sequence results showed the presence of a new subgroup in Pestivirus A species. This paper describes the nucleotide sequences of a new BVDV 1 (BVDV 1-v) subgroup member. |
Databáze: | OpenAIRE |
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