In Vitro Differentiation of Preosteoblast-Like Cells, MC3T3-E1, to Adipocytes Is Enhanced by 1,25(OH)2 Vitamin D3
| Autor: | Nalini Chandar, Elisha Pendleton |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Vitamin medicine.medical_specialty Tumor suppressor gene transdifferentiation Endocrinology Diabetes and Metabolism vitamin D Biology Calcitriol receptor lcsh:Diseases of the endocrine glands. Clinical endocrinology 03 medical and health sciences chemistry.chemical_compound Endocrinology Internal medicine Adipocyte medicine Vitamin D and neurology MC3T3 Rb1 Original Research lcsh:RC648-665 030102 biochemistry & molecular biology Mesenchymal stem cell osteoblasts Osteoblast 030104 developmental biology medicine.anatomical_structure chemistry Adipogenesis adipogenic differentiation |
| Zdroj: | Frontiers in Endocrinology, Vol 8 (2017) Frontiers in Endocrinology |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2017.00128/full |
| Popis: | Osteoblasts and adipocytes originate from common mesenchymal progenitor cells and are controlled by specific transcription factors. While 1,25-dihydroxyvitamin D3 (vitamin D) is known to be an important factor for osteoblast differentiation, there are conflicting reports regarding its effect on adipogenesis. In this study, we attempted to understand the effect of exposure of preosteoblasts (MC3T3-E1) to adipogenic media with and without vitamin D and determined the expression of adipogenic genes during this process. Our studies show that while transdifferentiation of preosteoblasts occurred on exposure to adipogenic media, the effect of vitamin D treatment was synergistic resulting in several hundred fold increase in adipocyte transcription factors C/EBPα and peroxisome proliferator-activated receptor-gamma (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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