Effect of female sex steroids on human endometrial CD16neg CD56bright natural killer cells
| Autor: | Kotaro Kitaya, Hideo Honjo, Shinji Fushiki, Takeshi Nakayama, Jinsuke Yasuda |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Cytotoxicity Immunologic medicine.medical_specialty medicine.medical_treatment chemical and pharmacologic phenomena Biology CD16 Endometrium Interferon-gamma Obstetrics and gynaecology Pregnancy Internal medicine Decidua medicine Humans Progesterone Hysterectomy Estradiol Tumor Necrosis Factor-alpha business.industry Receptors IgG Obstetrics and Gynecology hemic and immune systems medicine.disease CD56 Antigen Killer Cells Natural Cytolysis medicine.anatomical_structure Endocrinology Reproductive Medicine Female Cytokine secretion business Cell Division Thymidine |
| Zdroj: | Fertility and Sterility. 79:730-734 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(02)04818-5 |
| Popis: | Objective To clarify whether female sex steroids directly affect the bioactivity of the human endometrial CD16 neg CD56 bright natural killer (NK) cells. Design In vitro study. Setting University obstetrics and gynecology department. Patient(s) Thirteen women with histologically normal endometrium who were undergoing hysterectomy and seven women during the first trimester of pregnancy who were undergoing selective termination. Intervention(s) Endometrium or decidua was obtained. Main outcome measure(s) The effects of 17β-estradiol or progesterone (10 −6 , 10 −7 , and 10 −8 M) on the proliferation, cytolytic activity, and cytokine secretion of the isolated endometrial CD16 neg CD56 bright NK cells were examined using a 3 H-thymidine incorporation assay, 51 Cr-releasing assay, and enzyme-linked immunosorbent assay, respectively. Result(s) Neither 17β-estradiol nor progesterone had significant effects on the proliferation, cytolytic activity, and cytokine secretion of endometrial CD16 neg CD56 bright NK cells. Conclusion(s) Female sex steroids do not directly affect the bioactivity of the human endometrial CD16 neg CD56 bright NK cells. |
| Databáze: | OpenAIRE |
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