Assessing the partners of the RBP9-mRNP complex in Trypanosoma cruzi using shotgun proteomics and RNA-seq
| Autor: | Lysangela R. Alves, Bruno Accioly Alves Romagnoli, Marlon D.M. Santos, Helisa H. Wippel, Newton Medeiros Vidal, Paulo C. Carvalho, Samuel Goldenberg, Bruna H. Marcon, Jimena Ferreira da Costa, Alexandre Haruo Inoue |
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| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0301 basic medicine Trypanosoma cruzi Protozoan Proteins Sequence Homology RNA-binding protein RNA-Seq Computational biology Biology 03 medical and health sciences Translational regulation Transcriptional regulation Amino Acid Sequence Shotgun proteomics Molecular Biology Gene Regulation of gene expression High-Throughput Nucleotide Sequencing RNA-Binding Proteins Cell Biology biology.organism_classification 030104 developmental biology Gene Expression Regulation Ribonucleoproteins Research Paper |
| Zdroj: | RNA Biology. :1-13 |
| ISSN: | 1555-8584 1547-6286 |
| Popis: | Gene expression regulation in trypanosomes differs from other eukaryotes due to absence of transcriptional regulation for most of their genes. RNA-binding proteins (RBPs) associate with mRNAs and other regulatory proteins to form ribonucleoprotein complexes (mRNPs), which play a major role in post-transcriptional regulation. Here, we show that RBP9 is a cytoplasmic RBP in Trypanosoma cruzi with one RNA-recognition motif (RRM). The RBP9 sedimentation profile in a sucrose gradient indicated its presence in cytoplasmic translational complexes, suggesting its involvement in translation regulation. Taking this result as a motivation, we used shotgun proteomics and RNA-seq approaches to assess the core of the RBP9-mRNP complex. In epimastigotes in exponential growth, the complex was composed mostly by RBPs involved in RNA metabolism, such as ZC3H39, UBP1/2, NRBD1, and ALBA3/4. When parasites were subjected to nutritional stress, our analysis identified regulatory RBPs and the translation initiation factors eIF4E5, eIF4G5, eIF4G1, and eIF4G4. The RNA-seq results showed that RBP9-mRNP complex regulates transcripts encoding some RBPs – e.g. RBP5, RBP6, and RBP10 -, and proteins involved in metabolic processes. Therefore, we argue that RBP9 is part of cytoplasmic mRNPs complexes associated with mRNA metabolism and translation regulation in T. cruzi. |
| Databáze: | OpenAIRE |
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