The coordinated action of RNase III and RNase G controls enolase expression in response to oxygen availability in Escherichia coli
| Autor: | Min-Ho Lee, Ji-Hyun Yeom, Hyun-Lee Kim, Nam-Chul Ha, Jaejin Lee, Se-Hoon Sim, Minkyung Ryu, Minji Sim, Minju Joo, Kangseok Lee, Jang-Cheon Cho, Yoonsoo Hahn |
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| Rok vydání: | 2019 |
| Předmět: |
Ribonuclease III
0301 basic medicine Untranslated region RNase P Endoribonuclease activity 030106 microbiology lcsh:Medicine Gene Expression Microbiology Article 03 medical and health sciences Bacterial genetics Endoribonucleases Escherichia coli Protein biosynthesis RNA Messenger RNA Processing Post-Transcriptional lcsh:Science Regulation of gene expression Messenger RNA Multidisciplinary biology Chemistry Escherichia coli Proteins lcsh:R RNA Gene Expression Regulation Bacterial Oxygen RNA Bacterial 030104 developmental biology Biochemistry Phosphopyruvate Hydratase biology.protein lcsh:Q Microbial genetics |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-53883-y |
| Popis: | Rapid modulation of RNA function by endoribonucleases during physiological responses to environmental changes is known to be an effective bacterial biochemical adaptation. We report a molecular mechanism underlying the regulation of enolase (eno) expression by two endoribonucleases, RNase G and RNase III, the expression levels of which are modulated by oxygen availability in Escherichia coli. Analyses of transcriptional eno-cat fusion constructs strongly suggested the existence of cis-acting elements in the eno 5′ untranslated region that respond to RNase III and RNase G cellular concentrations. Primer extension and S1 nuclease mapping analyses of eno mRNA in vivo identified three eno mRNA transcripts that are generated in a manner dependent on RNase III expression, one of which was found to accumulate in rng-deleted cells. Moreover, our data suggested that RNase III-mediated cleavage of primary eno mRNA transcripts enhanced Eno protein production, a process that involved putative cis-antisense RNA. We found that decreased RNase G protein abundance coincided with enhanced RNase III expression in E. coli grown anaerobically, leading to enhanced eno expression. Thereby, this posttranscriptional up-regulation of eno expression helps E. coli cells adjust their physiological reactions to oxygen-deficient metabolic modes. Our results revealed a molecular network of coordinated endoribonuclease activity that post-transcriptionally modulates the expression of Eno, a key enzyme in glycolysis. |
| Databáze: | OpenAIRE |
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