The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster
| Autor: | Shreyas Jois, Soeren Diegelmann, Annika Jansen, Henrike Scholz, Laura Velo Escarcena, Katharina Kastenholz, Nicole Strudthoff |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Food intake Molecular signaling General Immunology and Microbiology biology ved/biology General Chemical Engineering General Neuroscience ved/biology.organism_classification_rank.species Liquid food Food consumption Computational biology biology.organism_classification General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 030104 developmental biology Feeding behavior Melanogaster Food science Drosophila melanogaster Model organism |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of the right type and quantity of food therefore has a major influence on quality of life. Research on feeding behavior focuses on the underlying processes that ensure actual feeding and unravels the role of factors regulating internal energy homeostasis and the neuronal bases of decision-making. The model organism Drosophila melanogaster, with its great variety of genetically traceable tools for labeling and manipulating single neurons, allows mapping of neuronal networks and identification of molecular signaling cascades involved in the regulation of food intake. This report demonstrates the CApillary FEeder assay (CAFE) and shows how to measure food intake in a group of flies for time spans ranging from hours to days. This easy-to-use assay consists of glass capillaries filled with liquid food that flies can freely access and feed on. Food consumption in the assay is accurately determined using simple measurement tools. Herein we describe step-by-step the method from setup to successful execution of the CAFE assay, and provide practical examples to analyze the food intake of a group of flies under controlled conditions. The reader is guided through possible limitations of the assay, and advantages and disadvantages of the method compared to other feeding assays in D. melanogaster are evaluated. |
| Databáze: | OpenAIRE |
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