A small RNA complementary to an intervening sequence is produced late in SV40 infection
| Autor: | Luis P. Villarreal, Rodolfo Campos, Susan Jovanovich |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Genetics
DNA Replication Small RNA Multidisciplinary Base Sequence Intron DNA Recombinant RNA-dependent RNA polymerase RNA Nucleic Acid Hybridization Nuclease protection assay DNA Restriction Enzymes Simian virus 40 Biology Virus Replication RNA editing RNA splicing RNA Viral RNA Messenger Small nuclear RNA Plasmids |
| Zdroj: | Nature. 291(5813) |
| ISSN: | 0028-0836 |
| Popis: | The post-transcriptional modifications that affect simian virus 40 (SV40) mRNA are similar to those undergone by most eukaryotic mRNAs and include 5′-end capping, 3′ polyadenylation and splicing of mRNA precursors1. Late in SV40 infection unspliced 19S RNAs are found predominantly in the nucleus whereas the spliced 19S RNAs are mainly cytoplasmic2,3. Nuclear and cytoplasmic 19S RNAs differ in that all the cytoplasmic species have lost a 32-nucleotide intervening sequence located between 0.760 and 0.765 map units3,4. Previous transcriptional studies of SV40 deletion mutants implied that splicing of the late nuclear transcripts and transport of RNA into the cytoplasm were coupled events5. More recent studies, however, demonstrate that transport of unspliced 19S RNA can occur when the sequences encompassing the 32-nucleotide intervening sequence (0.760–0.765 map units) are deleted6. We have previously proposed that this small 19S RNA intervening sequence may be involved in the retention of the unspliced RNA within the nucleus3. One hypothesis to account for recognition of the 32-nucleotide intervening sequence by a nuclear retention system postulates the existence of a small nuclear RNA complementary to the intervening sequence. We describe here the initial characterization of a small nuclear RNA made late in SV40 infection which is complementary to the DNA of the 19S intervening sequence. Several host cytoplasmic RNAs also hybridize to this intervening sequence. |
| Databáze: | OpenAIRE |
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