GS-441524 inhibits African swine fever virus infection in vitro
| Autor: | Zhiying Xu, Qi Gao, Jiachen Zheng, Xiaojun Chen, Guihong Zhang, Yang Chen, Heng Wang, Zezhong Zheng, Runda Xu, Lang Gong, Shengxun Zhang, Xiongnan Chen, Zhao Huang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adenosine Transcription Genetic GTP' Swine 030106 microbiology Virus Replication Antiviral Agents African swine fever virus 03 medical and health sciences chemistry.chemical_compound Transcription (biology) Virology RNA polymerase Chlorocebus aethiops Macrophages Alveolar Animals African Swine Fever Vero Cells Pathogen Pharmacology biology DNA replication biology.organism_classification African Swine Fever Virus In vitro 030104 developmental biology Viral replication chemistry |
| Zdroj: | Antiviral Research. 191:105081 |
| ISSN: | 0166-3542 |
| Popis: | African swine fever virus (ASFV) is a highly infectious and lethal swine pathogen that causes serious socio-economic consequences in endemic countries for which no safe and effective vaccine is currently available. GS-441524, a 1-cyano-substituted adenine C-nucleoside ribose analogue, inhibits viral RNA transcription by competing with natural nucleosides (ATP, TTP, CTP, and GTP) and effectively inhibits viral RNA-dependent RNA polymerase activity. However, whether GS-441524 can inhibit the replication of DNA viruses is unknown. In this study, we confirmed that GS-441524 inhibits ASFV infection in porcine alveolar macrophages (PAMs) in a dose-dependent manner; GS-441524 significantly inhibited ASFV replication at different time points after ASFV infection, particularly at the early stages of viral replication. Notably, GS-441524 did not increase the levels of antiviral cytokines or ATP in PAMs. However, an increase in the concentration of natural ATP in PAMs promoted the replication of ASFV and attenuated the inhibitory effect of GS-441524 in a dose-dependent manner. Our results suggest that GS-441524 is an effective antiviral against ASFV. |
| Databáze: | OpenAIRE |
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