Mesenchymal stem cells alter the frequency and cytokine profile of natural killer cells in abortion‐prone mice
| Autor: | Seyed Mohammad Moazzeni, Shahab Shahgaldi, Fatemeh Rezaei Kahmini |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Abortion Habitual Physiology medicine.medical_treatment Clinical Biochemistry Biology Mesenchymal Stem Cell Transplantation CD49b Natural killer cell Flow cytometry Cell therapy Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Pregnancy Decidua Immune Tolerance medicine Animals Maternal-Fetal Exchange Mice Inbred BALB C medicine.diagnostic_test Mesenchymal stem cell Cell Biology medicine.disease Killer Cells Natural Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine Cellular Microenvironment Mice Inbred DBA 030220 oncology & carcinogenesis Immunology Mice Inbred CBA Cytokines Female Infiltration (medical) |
| Zdroj: | Journal of Cellular Physiology. 235:7214-7223 |
| ISSN: | 1097-4652 0021-9541 |
| DOI: | 10.1002/jcp.29620 |
| Popis: | Natural killer cells, which play a pivotal role in the establishment and maintenance of normal pregnancy, are the most abundant leukocytes at the fetomaternal interface that their subsets frequencies and cytokine profile are influential factors in the preservation of the decidual tolerogenic microenvironment. Any imbalance in NK cells' frequency and functions could be associated with pregnancy failure. Mesenchymal stem cells (MSCs) are shown to have immunomodulatory effects on NK cells and their cytokine profile. The purpose of this study is to evaluate the impact of MSCs therapy on the cytokine profiles and subpopulations of NK cells in a murine model of recurrent pregnancy loss. Adipose-derived MSCs were injected intraperitoneally to the abortion-prone mice on Day 4.5 of gestation. The abortion rate was determined after MSCs administration and the frequency and cytokine profiles of the different subsets of NK cells were determined using the flow cytometry. Our results showed that, in abortion-prone mice, the frequency of CD49b+ NK cells was significantly higher than normal pregnant mice that decreased after therapy. We also demonstrated that MSCs downregulated the production of IFN-γ and upregulated IL-4 and IL-10 production by uNK cells. These findings indicate that MSCs can decrease the infiltration of CD49b+ NK cells to the fetomaternal interface and modulate the cytokine profile of NK cells from inflammatory to tolerogenic profile and thereby improve the tolerogenic microenvironment at the fetomaternal interface in benefit of pregnancy maintenance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text