Distinct Chromatin Configurations Regulate the Initiation and the Maintenance of hGH Gene Expression
| Autor: | Yugong Ho, Nancy E. Cooke, Brian M. Shewchuk, Stephen A. Liebhaber |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Transcriptional Activation
Cellular differentiation Mice Transgenic Locus (genetics) RNA polymerase II Biology Mice Gene expression Animals Deoxyribonuclease I Humans Gene Silencing Transgenes Promoter Regions Genetic Molecular Biology Gene Locus control region Sequence Deletion Genetics Regulation of gene expression Binding Sites Gene Expression Regulation Developmental Acetylation Articles DNA Polymerase II Cell Biology Locus Control Region Chromatin Growth Hormone Pituitary Gland embryonic structures biology.protein Transcription Factor Pit-1 |
| Zdroj: | Molecular and Cellular Biology. 33:1723-1734 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.01166-12 |
| Popis: | For many mammalian genes, initiation of transcription during embryonic development must be subsequently sustained over extensive periods of adult life. It remains unclear whether maintenance of gene expression reflects the same set of pathways as are involved in initial gene activation. The human pituitary growth hormone (hGH-N) locus is activated in the differentiating somatotrope midway through embryogenesis by a multicomponent locus control region (LCR). DNase I-hypersensitive site I (HSI) of the LCR is essential to full developmental activation of the hGH-N locus. Here we demonstrate that conditional deletion of HSI from the active hGH locus in the adult pituitary effectively silences hGH-N expression. Analyses of chromatin structure and locus positioning demonstrate that a specific subset of the HSI functions active in the embryo retain their HSI dependence in the adult pituitary. These functions sustain engagement of the hGH locus with polymerase II (Pol II) factories, histone acetylation at the hGH-N promoter, and looping of the LCR to its target promoter. These data reveal that HSI is essential to both the maintenance and the initiation phases of gene expression. These observations contribute to our mechanistic understanding of how stable patterns of mammalian gene expression are established in a terminally differentiated cell. |
| Databáze: | OpenAIRE |
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