PSA-NCAM is required for activity-induced synaptic plasticity
| Autor: | Cheng Wang, Dominique Muller, Galina Skibo, Nicolas Toni, V. Calaora, Geneviève Rougon, Harold Cremer, Jozsef Zoltan Kiss |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Neuronal Plasticity/drug effects/ physiology
Glycoside Hydrolases N-Acetylneuraminic Acid/analysis/ physiology Neuroscience(all) Long-Term Potentiation Neural Inhibition/drug effects/physiology Biology Hippocampal formation Hippocampus Receptors N-Methyl-D-Aspartate Neural Cell Adhesion Molecules/analysis/ physiology Synapse Rats Sprague-Dawley Hippocampus/chemistry/physiology Mice Organ Culture Techniques medicine Premovement neuronal activity Animals Microscopy Immunoelectron Neural Cell Adhesion Molecules Neuronal Plasticity Polysialic acid General Neuroscience Long-term potentiation Neural Inhibition Bicuculline Immunohistochemistry Long-Term Potentiation/drug effects/physiology Mice Mutant Strains N-Acetylneuraminic Acid Cell biology ddc:616.8 Synapses/chemistry/physiology/ultrastructure Rats Electrophysiology nervous system Animals Newborn Glycoside Hydrolases/pharmacology Synaptic plasticity Synapses Receptors N-Methyl-D-Aspartate/physiology Neural cell adhesion molecule Neuroscience medicine.drug |
| Zdroj: | Neuron, Vol. 17, No 3 (1996) pp. 413-422 |
| ISSN: | 0896-6273 |
| Popis: | Hippocampal organotypic slice cultures maintained 10–20 days in vitro express a high level of the polysialylated embryonic form of neural cell adhesion molecule (NCAM) (PSA–NCAM). Treatment of the cultures with endoneuraminidase-N selectively removed polysialic acid (PSA) from NCAM and completely prevented induction of long-term potentiation (LTP) and long-term depression (LTD) without affecting cellular or synaptic parameters. Similarly, slices prepared from transgenic mice lacking the NCAM gene exhibited a decaying LTP. No inhibition of N-methyl-D-aspartic acid receptor–dependent synaptic responses was detected. Washout of the enzyme resulted in reexpression of PSA immunoreactivity which correlated with a complete recovery of LTP and LTD. This reexpression was blocked by TTX and low calcium and enhanced by bicuculline. Taken together, these results indicate that neuronal activity regulates the expression of PSA–NCAM at the synapse and that this expression is required for the induction of synaptic plasticity. |
| Databáze: | OpenAIRE |
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