Full length cDNA of rat RT1.DMa and RT1.DMb and expression of RT1.DM genes in dendritic and Langerhans cells
| Autor: | Jürgen Neumann, Christoph Scheicher, Deming Sun, Uwe Wendling, Konrad Reske, Maria Mehlig, Detlev Kurth, Karin Demleitner, Jörg Hildmann |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
endocrine system DNA Complementary Transcription Genetic medicine.drug_class Clinical Biochemistry Blotting Western Genes MHC Class II Molecular Sequence Data Gene Expression Bone Marrow Cells Monoclonal antibody Major histocompatibility complex Biochemistry Islets of Langerhans Histocompatibility Antigens Gene expression medicine Animals Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence Cells Cultured MHC class II biology Base Sequence Chemistry Antibodies Monoclonal Dendritic Cells Blotting Northern Molecular biology Rats Blot Rats Inbred Lew biology.protein Beta protein Antibody |
| Zdroj: | Biological chemistry. 378(9) |
| ISSN: | 1431-6730 |
| Popis: | MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA clones coding for the subunits alpha and beta of rat DM molecules as well as a mAb directed against the luminal moiety of the beta subunit. Here we describe the establishment of the first full length cDNA clones of rat RT1.DMa and RT1.DMb. The mode of expression of RT1.DM at the transcript level in bone marrow culture-derived dendritic cells, in Langerhans cells and in a number of additional accessory cells is reported. The beta protein was identified in detergent lysates of RT1.DM expressing cells by Western blot analysis using a newly established monoclonal antibody directed against the luminal part of RT1.DMbeta. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|