5-aminosalicylic acid interferes in the cell cycle of colorectal cancer cells and induces cell death modes
| Autor: | Kamila Wolanin, Pim J. Koelink, Marij A C Mieremet-Ooms, Hein W. Verspaget, Cornelis B.H.W. Lamers, Whillem E. Corver, Daniel W. Hommes |
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| Rok vydání: | 2010 |
| Předmět: |
G2 Phase
Mitotic index Cell cycle checkpoint Colorectal cancer Survivin Mitosis Apoptosis Spindle Apparatus Biology Inhibitor of Apoptosis Proteins S Phase medicine Humans Immunology and Allergy Mesalamine Mitotic catastrophe Anti-Inflammatory Agents Non-Steroidal Gastroenterology 5-ASA apoptosis mitotic catastrophe cell death colorectal cancer activated-receptor-gamma flow-cytometry mitotic catastrophe carcinoma cells ppar-gamma inhibition apoptosis colon growth risk Cell cycle Flow Cytometry HCT116 Cells medicine.disease digestive system diseases PPAR gamma Immunology Cancer research Spindle organization Caco-2 Cells Colorectal Neoplasms HT29 Cells Microtubule-Associated Proteins Cell Division |
| Zdroj: | Inflammatory Bowel Diseases, 16(3), 379-389 |
| ISSN: | 1078-0998 |
| DOI: | 10.1002/ibd.21086 |
| Popis: | Introduction: Epidemiological data suggests that 5-aminosalicylic acid (5-ASA), a nonsteroidal antiinflammatory drug used in the treatment of inflammatory bowel diseases, prevents colorectal cancer development in these patients, although the mechanisms remain incompletely understood. Methods and Results: Here we report that 5-ASA prevents growth of several colorectal cancer cell lines by interfering in the cell cycle, i.e., an S-phase and G2/M phase arrest, dependent on 5-ASA dosage and concentration, together with an increased mitotic index. In addition, prolonged cell cycle arrest by repeated 5-ASA treatment induced apoptosis and caused abnormal spindle organization leading to mitotic catastrophe, another form of cell death. Conclusion: These observations illustrate that 5-ASA has chemopreventive and chemotherapeutic properties. (Inflamm Bowel Dis 2010;) |
| Databáze: | OpenAIRE |
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