Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo-Implications for biomarker discovery

Autor: Diána Kitka, Sakari Joenväärä, Maarit Takatalo, Zoltán Varga, Maija Puhka, Maarit Neuvonen, Risto Renkonen, Mayank Saraswat, Pia Siljander, Rienk Nieuwland, Mari Palviainen
Přispěvatelé: Laboratory Specialized Diagnostics & Research, ACS - Microcirculation, Extracellular Vesicles, Helsinki One Health (HOH), Division of Pharmaceutical Biosciences, Molecular and Integrative Biosciences Research Programme, Drug Research Program, University of Helsinki, HUSLAB, Transplantation Laboratory, Helsinki University Hospital Area, Institute for Molecular Medicine Finland, Department of Pathology, Department of Bacteriology and Immunology, Infection Biology Research Program, Risto Renkonen / Principal Investigator, HUS Head and Neck Center, Biochemistry and Biotechnology
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Serum Proteins
Proteome
Physiology
Proteomes
Protein Corona
Biochemistry
Mass Spectrometry
0302 clinical medicine
Animal Cells
Immune Physiology
BINDING
Blood plasma
NANOPARTICLES
Medicine and Health Sciences
Platelet
PROTEIN-CORONA
Multidisciplinary
Immune System Proteins
biology
medicine.diagnostic_test
Chemistry
MICROPARTICLES
Gene Ontologies
Blood Proteins
Genomics
Middle Aged
Flow Cytometry
Blood proteins
Healthy Volunteers
Body Fluids
Blood
030220 oncology & carcinogenesis
Medicine
Female
Antibody
Anatomy
Cellular Types
Research Article
Adult
Blood Platelets
Platelets
SURFACE
Protein mass spectrometry
Science
Immunology
Blood Plasma
Antibodies
Acid-citrate-dextrose
Flow cytometry
03 medical and health sciences
Extracellular Vesicles
medicine
Genetics
Humans
EXOSOMES
Blood Cells
Plasma Proteins
Biology and Life Sciences
Proteins
Computational Biology
Cell Biology
Genome Analysis
PREANALYTICAL PARAMETERS
SIZE
030104 developmental biology
biology.protein
BLOOD-PLASMA
VISUALIZATION
3111 Biomedicine
Biomarkers
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 8, p e0236439 (2020)
PLoS ONE, 15(8 August):e0236439. Public Library of Science
ISSN: 1932-6203
Popis: Extracellular vesicles (EVs) in human blood are a potential source of biomarkers. To which extent anticoagulation affects their concentration, cellular origin and protein composition is largely unexplored. To study this, blood from 23 healthy subjects was collected in acid citrate dextrose (ACD), citrate or EDTA, or without anticoagulation to obtain serum. EVs were isolated by ultracentrifugation or by size-exclusion chromatography (SEC) for fluorescence-SEC. EVs were analyzed by micro flow cytometry, NTA, TEM, Western blot, and protein mass spectrometry. The plasma EV concentration was unaffected by anticoagulants, but serum contained more platelet EVs. The protein composition of plasma EVs differed between anticoagulants, and between plasma and serum. Comparison to other studies further revealed that the shared EV protein composition resembles the "protein corona" of synthetic nanoparticles incubated in plasma or serum. In conclusion, we have validated a higher concentration of platelet EVs in serum than plasma by contemporary EV methods. Anticoagulation should be carefully described (i) to enable study comparison, (ii) to utilize available sample cohorts, and (iii) when preparing/selecting biobank samples. Further, the similarity of the EV protein corona and that of nanoparticles implicates that EVs carry both intravesicular and extravesicular cargo, which will expand their applicability for biomarker discovery.
Databáze: OpenAIRE
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