Applications of fluorophore-containing microbial growth media
| Autor: | Milton M. Takeguchi, Ralph A. Messing, E D Nowlan, Lynn B. Simpson, William S. Ramsey |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Staphylococcus aureus
Fluorophore food.ingredient Bacterial growth Sulfonic acid medicine.disease_cause Applied Microbiology and Biotechnology Anilino Naphthalenesulfonates Fluorescence Cell wall chemistry.chemical_compound food Species Specificity Cell Wall medicine Escherichia coli Agar chemistry.chemical_classification Bacteriological Techniques Ecology biology Bacteria biology.organism_classification Culture Media chemistry Biochemistry Food Science Biotechnology Research Article |
| Zdroj: | Applied and environmental microbiology. 39(2) |
| ISSN: | 0099-2240 |
| Popis: | Media containing the fluorogenic compound 8-anilino-1-naphthalene sulfonic acid may be used to discriminate between gram-positive and gram-negative bacteria and to differentiate between various species of bacteria. Fluorescent light emitted from colonies of gram-negative bacteria on 8-anilino-1-naphthalene sulfonic acid-containing agar was visually more intense than that on gram-positive bacteria. The emitted light from the gram-negative bacteria differed in wave-lengths from that of light emitted by colonies of gram-positive bacteria. The fluorescent intensity of colonies on complete 8-anilino-1-napthalene sulfonic acid agar supplemented with 1% of single substrates varied depending on the bacterial species, thus allowing the development of profiles used to identify 12 different species. |
| Databáze: | OpenAIRE |
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