Q192R variant in paraoxonase 1 gene confers susceptibility to leiomyoma
| Autor: | Neda Fatahi, Soghra Zarei, Mahnaz Torfeh, Shirin Shahbazi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
0106 biological sciences 0301 basic medicine medicine.medical_specialty Genotype Single-nucleotide polymorphism Polymorphism Single Nucleotide 01 natural sciences 03 medical and health sciences Gene Frequency Internal medicine medicine Humans Genetic Predisposition to Disease Radiology Nuclear Medicine and imaging Uterine leiomyoma Leiomyoma biology Aryldialkylphosphatase business.industry Paraoxonase Myoma Heterozygote advantage General Medicine medicine.disease PON1 030104 developmental biology Endocrinology Oncology Case-Control Studies Uterine Neoplasms biology.protein Female business 010606 plant biology & botany |
| Zdroj: | Journal of Cancer Research and Therapeutics. 16:884 |
| ISSN: | 0973-1482 |
| DOI: | 10.4103/jcrt.jcrt_923_16 |
| Popis: | Objective Paraoxonase 1 (PON1) plays a defensive role against oxidative stress by destroying oxidized lipids. Q192R single nucleotide polymorphism of PON1 gene alters the enzyme's activity. Several investigations reported a link between Q192R and an increased risk of developing tumors including uterine leiomyomas. We assessed the antioxidant effects of Q192R on myoma which fluctuate in frequency between populations. Study Design The cohort consisted of 68 unrelated uterine leiomyoma patients and 93 healthy controls that were randomly selected from women with no ultrasonographic evidence of myoma. Materials and Methods Genotyping was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction. Chi-square test was selected to evaluate differences between the groups. Results To analyze the correlation between PON1 Q192R and leiomyoma risk, the AA genotype was given as a reference genotype then the two other genotypes were compared with the reference. A significantly (P < 0.05) increased risk of myoma was observed with both Q192R homozygote GG and heterozygote AG genotypes. The odds ratio (OR) of AG genotype was calculated 1.8 (confidence interval [CI]: 0.94-3.62). A higher OR was seen with GG genotype (OR: 2.8; 95% CI: 0.98-8.18). Conclusion Oxidative stress has been suspected of having a link with tumor development, and the role of endogenous-free radical scavenger is taken into consideration. Increased protein oxidative stress status and reduced antioxidant capacity have been observed in leiomyomas patients. Our study indicates that the low-antioxidant PON1 R192 allele correlates to leiomyoma development. |
| Databáze: | OpenAIRE |
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