Assessment of the effect of silica calcium phosphate nanocomposite on mesenchymal stromal cell differentiation and bone regeneration in critical size defect
| Autor: | Nihal AlMuraikhi, A. Y. Al-Zahrani, Mohammed Alkindi, Sarah BinHamdan, Shams Altwaim |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Stromal cell
Regenerative medicine 030207 dermatology & venereal diseases 03 medical and health sciences Craniofacial 0302 clinical medicine medicine Regeneration Bone regeneration Bone General Dentistry Cell ceramic Chemistry Regeneration (biology) Mesenchymal stem cell RK1-715 030206 dentistry Cell biology RUNX2 medicine.anatomical_structure Dentistry Medicine Original Article Bone marrow Stem cell Reconstruction |
| Zdroj: | The Saudi Dental Journal Saudi Dental Journal, Vol 33, Iss 8, Pp 1119-1125 (2021) |
| ISSN: | 1658-3558 1013-9052 |
| Popis: | Objective The research was designed to assess silica calcium phosphate nanocomposite (SCPC) biocompatibility and bioactivity as an osteoinductive scaffold and cell carrier. Consequently, the ability of cell seeded SCPC implant to regenerate a critical size defect in rat calvarium. Materials and Methods The study was conducted in two parts. A series of in vitro experiments on bone marrow stromal cells (MSCs) seeded in the SCPC scaffold evaluated cell attachment, proliferation and osteogenic differentiation. In the second part, a cell seeded SCPC construct was implanted in rat calvarium and bone regeneration was assessed by histological examination to evaluate the newly formed bone quality and the residual graft volume. Results In vitro experimentation revealed that MSCs cultured on SCPC maintained viability and proliferation when seeded into the SCPC. Scanning electron microscopy demonstrated cell adhesion and calcium appetite formation, MSCs differentiated towards the osteogenic lineage as indicated by the upregulation of RUNX2, ALP, Col1a1 markers. Histological examination showed regeneration from the periphery and core of the defect with new bone formation at different stages of maturation. Conclusion Regenerative medicine delivers promising solutions and technologies for application in craniofacial reconstruction. SCPC scaffold has the potential to be used as a cell carrier to achieve stem cell-based bone regeneration, which provides a viable alternative for treatment of challenging critical size defect. |
| Databáze: | OpenAIRE |
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