RETRACTED: Intracellular distribution of p300 and its differential recruitment to aggresomes in breast cancer
| Autor: | María Eugenia Fermento, Norberto Ariel Gandini, Juan E. Perez, Horacio V. Maturi, Alejandro Carlos Curino, Maria Marta Facchinetti, Cecilia A. Lang |
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| Rok vydání: | 2010 |
| Předmět: |
Cytoplasm
Proteasome Endopeptidase Complex Pathology medicine.medical_specialty Transcription Genetic Blotting Western Clinical Biochemistry Mammary gland Breast Neoplasms Mammary Neoplasms Animal Mice Transgenic Vimentin Adenocarcinoma Inclusion bodies Pathology and Forensic Medicine Mice Mammary Glands Animal Ubiquitin Reference Values Cell Line Tumor medicine Animals Humans Breast Molecular Biology Inclusion Bodies biology Cancer medicine.disease Immunohistochemistry Cell biology medicine.anatomical_structure biology.protein Female Breast disease E1A-Associated p300 Protein Intracellular Nuclear localization sequence |
| Zdroj: | Experimental and Molecular Pathology. 88:256-264 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/j.yexmp.2010.01.007 |
| Popis: | It has been recently suggested that p300 cytoplasmic redistribution and degradation are important for controlling the availability and activity of the protein as a transcriptional coactivator. As a step towards determining the functional relevance of p300 intracellular redistribution in mammary cancer, we aimed at studying p300 localization in two different animal models of mammary carcinoma as well as in human primary breast carcinoma samples. Analysis of p300 protein levels showed stronger expression in tumor epithelia than in normal mammary gland. Cytoplasmic localization of p300 was observed in malignant cells. Furthermore, cytoplasmic p300 was found in tumor epithelia whereas nuclear localization was observed in normal mammary glands in both animal models and in non-malignant adjacent areas of human breast cancer specimens. Interestingly, proteasomal inhibition induced p300 redistribution to perinuclear inclusion bodies in tumor but not in normal mammary gland-derived cells. These inclusions were confirmed to be aggresomes by doing immunofluorescence for ubiquitin, vimentin and 20S proteasomal subunit. Taken together, these findings show that both the localization of p300 and the recruitment to aggresomes differ between mammary tumors and normal mammary glands, and suggest that the formation of these inclusions could be a potential target for therapeutic intervention. |
| Databáze: | OpenAIRE |
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