Molecular detection of a bacterial contaminant Bacillus pumilus in symptomless potato plant tissue cultures
| Autor: | JF Hutchinson, Daniel Isenegger, M. Barlass, Paul J. Taylor, G. R. McGregor, K. Mullins |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Plant tissue culture Molecular Sequence Data Bacillus Plant Science Biology Polymerase Chain Reaction Sensitivity and Specificity Microbiology law.invention Tissue culture law Culture Techniques RNA Ribosomal 16S Cleaved amplified polymorphic sequence Polymerase chain reaction Plant Diseases Solanum tuberosum Base Sequence Bacillus pumilus fungi food and beverages Sequence Analysis DNA General Medicine biology.organism_classification Molecular biology Random Amplified Polymorphic DNA Technique RAPD genomic DNA DNA profiling Agronomy and Crop Science Polymorphism Restriction Fragment Length |
| Zdroj: | Plant Cell Reports. 21:814-820 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-003-0583-z |
| Popis: | An aberrant random amplified polymorphic DNA (RAPD) marker in genomic DNA of tissue culture plantlets was frequently observed during a comparison of DNA fingerprints derived from potato germplasm grown in tissue culture and the field. The RAPD marker was cloned, sequenced and determined to be of bacterial origin. A bacterial contaminant was isolated from the tissue culture plants and identified as a Bacillus pumilus. A set of sequence characterised amplified region (SCAR) primers were designed from the sequence of the cloned fragment and tested for the specific detection of B. pumilus. Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) were also used to generate B. pumilus profiles specific to our isolate in order to test and confirm the sequence homology of amplified markers generated from a range of DNA samples isolated from tissue culture plants and pure isolates of B. pumilus-like bacteria. |
| Databáze: | OpenAIRE |
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