Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins
Autor: | Su Chul Jang, Cecilia Lässer, Rienk Nieuwland, Aleksander Cvjetkovic, Rossella Crescitelli, Mohammad Ali Hosseinpour Feizi, Jan Lötvall, Nasibeh Karimi |
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Přispěvatelé: | ACS - Amsterdam Cardiovascular Sciences, Laboratory Specialized Diagnostics & Research, ACS - Microcirculation |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Proteomics Serum Proteome Lipoproteins Blotting Western Exosomes 03 medical and health sciences Cellular and Molecular Neuroscience Plasma 0302 clinical medicine Size-exclusion chromatography Humans Biomarker discovery Molecular Biology Pharmacology Mass spectrometry Chemistry Vesicle Cell Biology Extracellular vesicle Blood Proteins Extracellular vesicles Blood proteins Microvesicles Microscopy Electron 030104 developmental biology Biochemistry 030220 oncology & carcinogenesis Chromatography Gel Molecular Medicine Original Article Density cushion Biomarkers Lipoprotein |
Zdroj: | Cellular and Molecular Life Sciences Cellular and molecular life sciences, 75(15), 2873-2886. Birkhauser Verlag Basel |
ISSN: | 1420-9071 1420-682X |
Popis: | The isolation of extracellular vesicles (EVs) from blood is of great importance to understand the biological role of circulating EVs and to develop EVs as biomarkers of disease. Due to the concurrent presence of lipoprotein particles, however, blood is one of the most difficult body fluids to isolate EVs from. The aim of this study was to develop a robust method to isolate and characterise EVs from blood with minimal contamination by plasma proteins and lipoprotein particles. Plasma and serum were collected from healthy subjects, and EVs were isolated by size-exclusion chromatography (SEC), with most particles being present in fractions 8–12, while the bulk of the plasma proteins was present in fractions 11–28. Vesicle markers peaked in fractions 7–11; however, the same fractions also contained lipoprotein particles. The purity of EVs was improved by combining a density cushion with SEC to further separate lipoprotein particles from the vesicles, which reduced the contamination of lipoprotein particles by 100-fold. Using this novel isolation procedure, a total of 1187 proteins were identified in plasma EVs by mass spectrometry, of which several proteins are known as EV-associated proteins but have hitherto not been identified in the previous proteomic studies of plasma EVs. This study shows that SEC alone is unable to completely separate plasma EVs from lipoprotein particles. However, combining SEC with a density cushion significantly improved the separation of EVs from lipoproteins and allowed for a detailed analysis of the proteome of plasma EVs, thus making blood a viable source for EV biomarker discovery. Electronic supplementary material The online version of this article (10.1007/s00018-018-2773-4) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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