Role of Nicotinic Acetylcholine Receptor α3 and α7 Subunits in Detrusor Overactivity Induced by Partial Bladder Outlet Obstruction in Rats
| Autor: | Jun Sung Koh, Joon Chul Kim, Wang Jin Park, Eun-Young Park, Tae-Kon Hwang, Hyo Sin Kim |
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| Rok vydání: | 2015 |
| Předmět: |
Detrusor muscle
medicine.medical_specialty Contraction (grammar) Urology Alpha7 Nicotinic Acetylcholine Receptor lcsh:RC870-923 urologic and male genital diseases Bladder outlet obstruction chemistry.chemical_compound medicine Urothelium Methyllycaconitine Urinary Bladder Overactive business.industry Urinary bladder neck obstruction lcsh:Diseases of the genitourinary system. Urology medicine.disease Urinary Bladder Neck Obstruction Nicotinic acetylcholine receptor medicine.anatomical_structure Neurology chemistry Original Article Hexamethonium Neurology (clinical) business |
| Zdroj: | International Neurourology Journal, Vol 19, Iss 1, Pp 12-18 (2015) International Neurourology Journal |
| ISSN: | 2093-6931 |
| DOI: | 10.5213/inj.2015.19.1.12 |
| Popis: | Purpose: To investigate the role of α3 and α7 nicotinic acetylcholine receptor subunits (nAChRs) in the bladder, using a rat model with detrusor overactivity induced by partial bladder outlet obstruction (BOO). Methods: Forty Sprague-Dawley rats were used: 10 were sham-operated (control group) and 30 were observed for 3 weeks after partial BOO. BOO-induced rats were further divided into 3 groups: Two groups of 10 rats each received intravesicular infusions with hexamethonium (HM group; n=10) or methyllycaconitine (MLC group; n=10), which are antagonists for α3 and α7 nAChRs, respectively. The remaining BOO-induced rats received only saline infusion (BOO group; n=10). Based on the contraction interval measurements using cystometrogram, the contraction pressure and nonvoiding bladder contractions were compared between the control and the three BOO-induced groups. Immunofluorescent staining and Western blotting were used to analyze α3 and α7 nAChRs levels. Results: The contraction interval of the MLC group was higher than that of the BOO group (P |
| Databáze: | OpenAIRE |
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